BIO 3124 Study Guide - Midterm Guide: Treponema, Fetus, Globus Pallidus

1. What would a gram positive bacteria look like if you forgotthe alcohol step during the Gram stain procedure? Why?

2. What would a gram negative bacteria look like if you forgotthe alcohol step during the Gram stain procedure? Why?

3. What would a gram positive bacteria look like if you forgotthe safranin step during the Gram stain procedure? Why?

4. What would a gram negative bacteria look like if you forgotthe safranin step during the Gram stain procedure? Why?

Thank you!!

Microbiology quiz

True or False: Helicobacter pylori is the MOST common singleorganism found in the stomach.

True

False

Which of the following is NOT found in the gastrointestinaltract of a healthyindividual

protists

Clostridium

Bacteroides

Bacteroidetes

Which of the following is NOT produced by microorganisms in thegut of humans?

vitamin B12

vitamin K

vitamin C

CO2

Bacteria make up about ________ the weight of fecal matter.

one-fourth

one-third

one-half

one-fifth

Which of the following will happen after a person takes anantibiotic orally?

The growth of normal flora will be inhibited.

The growth of pathogens will be inhibited.

Opportunistic microorganisms such as antibiotic-resistantStaphylococcus will become established.

all of the above

Healthcare-associated infections are also called

opportunistic pathogenic infections.

attenuated infections.

virulent infections.

nosocomial infections.

Which of the following healthcare procedures may unintentionallyintroduce microorganisms into the patient?

catheterization

surgery

spinal puncture

all of the above

_______ is the growth of microorganisms NOT normally presentwithin a host.

Disease

Virulence

Infection

Pathogenicity

Adherence factors include ALL of the following EXCEPT

capsule.

fimbriae.

flagella.

lipopolysaccharide

B. Fill in the table based on the two images shown: Image 1 Image 2 Gram reaction Shape of cells

1. 2. Fill in the following table describing the steps of the gram stain: Step Reagent Purpose of reagent time Color of gram Positive cells after this step Color of gram Negative cells after this step 1 2 3 4 Answer the following questions:

1. Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory? 2. Suppose distilled water was accidentally used instead of alcohol in the Gram stain procedure. What would be the color of gram positive and gram negative bacteria at the end of the procedure? 3. What is the purpose of the mordant?

4. Why do gram positive bacteria retain the primary stain? 5. Suppose you did a gram stain on a sample of bacteria, and you saw bacilli that were gram positive next to bacilli that looked gram negative. What are two ways you could interpret these results?