LSCI 204 Study Guide - Final Guide: Nonsense Mutation, Glutamine, Transfer Rna
Document Summary
Solutions to 7. 03 problem set 3: in order to suppress tag and taa nonsense mutations, it requires two different trna"s that have anti-codons mutated to cua and uua. Having two mutations in two different trna genes at the same time is as extremely unlikely as it is to get two back mutations at the same time: the possible mutations are: Amber mutation is tag. trna has to have the anti-codon cua. Gene that encodes this trna must have the sequence cta. Ochre mutation is taa. trna has to have the anti-codon uua. Gene that encodes this trna must have the sequence tta. This sequence, given the possible mutations, could have mutated from: This is a highly unlikely event, because ems generally does not cause frameshift mutations: because p1 was grown on his+ kanr strain, cotransductants selected for. Kanr are his+. cotransduction frequency = of # cotransduc tants transduct ants total.
