BIOL 200 Study Guide - Final Guide: Transcription Preinitiation Complex, Transcription Factor Ii E, Stamen
Document Summary
Bacterial restriction enzymes cut dna at specific sites. Dna can be amplified from this vector. Studying genes of interest: dna libraries: digest entire genomes, first: mechanically, second: restriction enzyme digests, sequences are kept in genomic libraries, one genome fragment per plasmid cdna libraries - represent mrna tissue specificity, abundance. Specialized vectors permit efficient expression in higher eukaryotic cells: transient transfection transfect cultured cells with a vector. Protein is expressed from cdna in plasmid dna: not all cells will take up the plasma, eventually all cells will get rid of the plasmid. No centromere (no faithful segregation) => loose plasmid: stable transfection (do not use the term transformation) vector contains a antibiotic resistance gene; therefore only cells which contain the vector will survive. Whenever cells lose the vector they die: when the vector is transfected into the host chromosome => becomes stable.