BIOL 200 Lecture 17: Lecture 17 BIOL 200
5 Nov 2014
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He focuses on c. elegans and studies what factors affect a cell"s decision to stop dividing or continue dividing; every single gene he"s hit has some or other relation to tumor growth. We can also study what that gene does when it"s not mutated: experiment: take bits of dna and introduce them into plasmids and insert these into e. coli to produce lots of little. They do this using an ori: polylinker: we can use a single cut using a restriction enzyme to open up the vector so that you can introduce a new dna into the polylinker sequence. Digestion of a vector with 2 different enzymes facilitates directional cloning so that the differentially digested dna fragments we are interested in can only insert in 1 orientation. A tail with a single- stranded poly t oligonucleotide. Rt uses this primer to initiate single-strand dna synthesis that is fully complementary to the mrna template.
