You are told to make a 1:5 dilution of a solution. You need a total volume of 10 mL of the diluted solution. How much sample and diluent will you use?

You have a 10x concentrated Auxin hormone solution. For your experiment, you need 500 mL of a 2x solution of Auxin. How would you make a 500 mL of 2x solution from 10x solution?

What plant hormone would be most likely to promote seed germination?

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Related questions

1. Youneed to prepare 200 uL of each protein at a concentration of 1mg/mLfor an upcoming experiment. Calculate the recipe to make eachdesired solution at the above concentration and volume. Do you haveenough materials to make each dilution? Note: Your diluent iswater.

a. Alpha-tubulin: you have 500 uL at 5mg/mL

b. TGFbeta: you have 300 uL at 2.4 mg/mL

c. Albumin: you have 150 uL at 1 mg/mL

d. ConA: you have 100 uL at 1.5 mg/mL

2. You have 1L of 10XSDS-PAGE Running Buffer and you need to make as much 1X runningbuffer as possible for the upcoming experiment. How much 1X runningbuffer can you make using the entire 1L of 10X running buffer?

goldpanda491

A)Describe how you would prepare a 1X TBE electrophoresis bufferusing the 10X TBE as a stock solution assuming you need a finalvolume of 800 ml.

B) Assume that a 0.5% (w/v) stock solution is available in thelab that you will use to make up three different 5 ml workingsolutions. The concentration of each of the working solutions is asfollows: 0.25%, 0.05%, and 0.001%. Calculate the volume of stocksolution and water that will be used for each dilution. Indicatewhich micropipette you would use to make each working solution.

C) Assume that you need to mix a 1 mL stock solution of 10Xrestriction buffer with the following final concentration: 600 mMTris, 100 mM MgCl, 70 mM DTT. Further assume that you have thefollowing solutions available to you: 2 M Tris, 1M MgCl, and 1.5 MDTT. Describe the preparation of the 10X restriction buffer showingall calculations. Describe how you would dilute this 10Xrestriction buffer to 2X with a volume of 50 microliters. Rememberthese volumes are much too small for volumetric flasks.

Standard Plate Count Assessment

You want to make lemonade from scratch. This means combining lemon juice from lemons, sugar and water. This is really a dilution problem like the protocol described. The lemon juice is 2 times more concentrated than you want it to be. How would you dilute the lemon juice accordingly? Your answer must include the volumes of lemon juice and water (diluent) that need to be mixed together and also what the total volume will be after the dilution.

1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml - 2 ml = 8 ml diluent.

A. Your friend wants to perform 1/10 and 1/100 dilutions of the concentrated lemon juice. The person does not understand what this means and asks you for help. How would you explain to her what this means? How should she perform these dilutions?

1/10: _____ ml lemon juice and ______ ml water

1/100: _____ ml lemon juice and ______ ml water

A. You take 1 mL of concentrate juice and add 9 mL of water. What dilution did you make here?

B. You take 10 mL of concentrated juice and add 90 mL of water.What dilution did you make here?

C. Were your answers in A and B the same or different? Explain your answer.