A protein biochemist attempted to determine the amino acid sequenceof a decapeptide. Use the results from the trypsin, chymotrypsin,and cyanogen bromide treatments to suggest the amino acid sequenceof this decapeptide.
Trypsin digestion gave two fragments, containing the followingresidues (not in order):
T1: Ala, Arg, Phe, Pro, Thr, Trp, Tyr
T2: Lys, Met, Val
Chymotrypsin digestion gave three fragments with the followingresidues (not in order):
CT1: Ala, Arg, Phe, Pro
CT2: Thr, Trp
CT3: Lys, Met, Tyr, Val
N-terminal analysis of the CT1 tetrapeptide indicated that theN-terminal amino acid was Ala.
The cyanogen bromide gave only one single amino acid, methionine,and a nonapeptide.
What is a possible sequence of the decapeptide? Use three-letterabbreviations in your answer, and add dashes betweenresidues
A protein biochemist attempted to determine the amino acid sequenceof a decapeptide. Use the results from the trypsin, chymotrypsin,and cyanogen bromide treatments to suggest the amino acid sequenceof this decapeptide.
Trypsin digestion gave two fragments, containing the followingresidues (not in order):
T1: Ala, Arg, Phe, Pro, Thr, Trp, Tyr
T2: Lys, Met, Val
Chymotrypsin digestion gave three fragments with the followingresidues (not in order):
CT1: Ala, Arg, Phe, Pro
CT2: Thr, Trp
CT3: Lys, Met, Tyr, Val
N-terminal analysis of the CT1 tetrapeptide indicated that theN-terminal amino acid was Ala.
The cyanogen bromide gave only one single amino acid, methionine,and a nonapeptide.
What is a possible sequence of the decapeptide? Use three-letterabbreviations in your answer, and add dashes betweenresidues
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