Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review the Scientific Method Tutorial (found the Course Contentsection of the classroom under the Science Learning Center link)and the Scientific Method lab (Lab 1), so that you can betterunderstand how to design an experiment. It may also be helpful foryou to review your textbook and Lab 4 (Enzymes). As you review Lab4, you will be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).
As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughly explain how the addition ofthe acidic fluid impacted the overall reaction process (thisinformation will help you to answer question 4).
Hint: Keep in mind thatthe acid will change the environmental conditions of the experiment(for example, a low pH value could change the shape of the activesite on the enzyme protein), without directly participating in thereaction.
LabMaterials
You may need thefollowing, depending on your experimental design:
Materials in your labkit:
Design an experiment in which you will test theeffect of an acidic fluid on enzymatic activity. (Recall: enzymesare proteins.) To complete this project, it may be useful for youto review the Scientific Method Tutorial (found the Course Contentsection of the classroom under the Science Learning Center link)and the Scientific Method lab (Lab 1), so that you can betterunderstand how to design an experiment. It may also be helpful foryou to review your textbook and Lab 4 (Enzymes). As you review Lab4, you will be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).
As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must also thoroughly explain how the addition ofthe acidic fluid impacted the overall reaction process (thisinformation will help you to answer question 4).
Hint: Keep in mind thatthe acid will change the environmental conditions of the experiment(for example, a low pH value could change the shape of the activesite on the enzyme protein), without directly participating in thereaction.
LabMaterials
You may need thefollowing, depending on your experimental design:
Materials in your labkit:
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