In your lab you have several mouse cell lines growing in culture, designated culture A through culture D. Some of these cell lines are transformed and others are not. For each of the descriptions below, tell whether the cell line is transformed or normal, and explain your choice. If it is transformed, make an educated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factors to the medium. If the cells are exposed to damaging radiation, the growth curve is unaffected. Many of the cells have abnormal numbers of chromosomes.
B. Cell line C grows quickly. The cells are impervious to the addition of serum to their medium. Upon exposure to damaging radiation, most of the cells undergo apoptosis.
C. Cell line D grows quickly and is impervious to DNA damaging radiation, entering cycle after cycle of mitosis even after large dosis of radiation. If transfected with DNA containing a gene for p53, these cells will undergo apoptosis.
In your lab you have several mouse cell lines growing in culture, designated culture A through culture D. Some of these cell lines are transformed and others are not. For each of the descriptions below, tell whether the cell line is transformed or normal, and explain your choice. If it is transformed, make an educated guess about the gene involved in the transformation.
A. Cell line A is insensitive to the addition of growth factors to the medium. If the cells are exposed to damaging radiation, the growth curve is unaffected. Many of the cells have abnormal numbers of chromosomes.
B. Cell line C grows quickly. The cells are impervious to the addition of serum to their medium. Upon exposure to damaging radiation, most of the cells undergo apoptosis.
C. Cell line D grows quickly and is impervious to DNA damaging radiation, entering cycle after cycle of mitosis even after large dosis of radiation. If transfected with DNA containing a gene for p53, these cells will undergo apoptosis.