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tanwombat865Lv1

15 Nov 2021

In F⁺ x F^- crosses, the F^-recipient is converted to a donor with very high frequency.However, it is rare for a recipient to become a donor inHfr x F^- crosses. Explain why.

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22 Apr 2023

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Related questions

Which forms of horizontal gene transfer involve a DNA molecule that brings with it intact genetic pathways for further replication, such that the recipient cell could now initiate horizontal transfer as a donor cell? Choose all that apply.

A. Post-translational modification

B. F' x F- conjugation

C. Hfr x F- conjugation

D. F+ x F- conjugation

E. Transformation

brownmole618

Why is it important that the donor cultures are grown to exponential phase, but the recipient cells can be in stationary phase?

A scientist performed a phage-mediated transduction using a new and uncharacterized E. coli phage. After using a range of different recipient strains and the one donor, she noticed that one particular gene, the tub gene, was transduced from the donor to the recipient strain with much higher frequency than any of the other chromosomal markers.

She wrote in her lab book âThe phage appears to be unable to move from the lysogenic to the lytic pathwayâ.

(a) Do you think she is right? Answer yes or no. You do not need to justify your answer. (1 mark)

(b) Can you propose a mechanism for the high-level transduction of the tub marker as result of the phage infection? You may draw diagrams as part of your answer. (5 marks)