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3 Apr 2019

Please read and answer the following question

One of the major uses of biotechnology is in treating or curing human diseases. Gene therapy attempts to alter or replace cells to better combat a given disease. For example, say a disease is caused by a mutated gene that causes a cell to function abnormally. A recombinant virus with a correct gene can infect a diseased cell with the incorrect gene and restore the cell’s normal function. Another example deals with cancer. Cancer cells have specific markers on the outside of the cells that can be ‘read’ by immune cells. So immune cells can be taken out of the body, primed for a specific cancer marker, grown to large numbers, then injected back into the body to fight the cancer cells.

One of the first steps in gene therapy is to identify and obtain the gene of interest. There are many ways to identify the correct gene.

There are many ways genes can be put into cells. By the use of viruses, restriction enzymes, transformation, liposomes, microinjection, etc. Each of these methods comes with pros and cons.

After going through the protocol to insert the gene of interest into the cell, how do you know the gene is actually in there? Is it in the right spot? Is it working?

A. give ONE way to identify a gene of interest in a cell.

B. Chose a method of gene delivery. Explain how the gene is introduced into the cell. Give TWO pros and TWO cons to this method

C. Give ONE method to determine if your gene of interest is inside the cell give a method that determines if the gene is in the cell AND is functioning properly).

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Lelia Lubowitz
Lelia LubowitzLv2
3 Apr 2019

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