Experiment 1: Effect of organic challenge on the efficacy of Milton fluid.
This experiment is designed to demonstrate two concepts. Firstly, that a disinfectant, like Milton Fluid, will kill microbial contaminants on surfaces such as glass. Secondly, to evaluate the effect of organic challenge on the ability of Milton to act as a disinfectant. Milton is 1% Sodium hypochlorite containing 16.5% Sodium Chloride.
Method
i. Label 3 universal bottles A, B and C.
ii. Pour milk into universals A and B until they are 1/3 full.
iii. Let stand for 5 mins.
iv. Pour milk down sink.
v. Clean universal A, but not B with detergent and bottle brush.
vi. Add Milton solution to all universals (A, B and C).
vii. Let stand for 15 mins.
viii. Pour Milton solution down the sink.
ix. Using aseptic technique, divide the nutrient broth provided between the 3 universals A, B and C.
x. Incubate overnight at 35 degrees Celsius.
Experiment 2: Antibiotic disk test (Perform antibiotics test assay).
1. Three test stains are provided with this test 1) E.coli, 2) Staphylococcus "albus" (epidermidis), 3) Bacillus subtilis; antibiotics are unless announced otherwise provided a) Penicillin, b) Cefoxitin, c) Ampicillin. Antibiotics b) and c) are provided as pre-soaked discs while a) is provided as 100mg/1 and 10mg/1 solution.
2. Open sterile swab without touching the cotton tip.
3. Open source culture using aseptic technique.
4. Dip cotton, tip of swab into broth.
5. Carefully 'paint' the entire surface of a sterile agar plate with the cotton bud to cover all areas of the plate. (This will grow up to become a lawn of bacteria.)
6. Label the bottom of the plate with the numbers 1 to 4 evenly spaced out around the table.
7. Sterilize a forceps using alcohol. Danger - check the location the fire blanket in the lab. Keep the alcohol at least 20cm away from Bunsen. If the alcohol container catches fire, cover with a lid and call a demonstrator.
8. Dip a sterile disc into the Penicillin 10mg/1 dilution, shake off excess.
9. Place this carefully onto the surface of the plate at the position marked 1.
10. Repeat for the 100mg/1 Penicillium solution, putting the disc at location 2.
11. Use a pre-soaked disc Cefoxitin. For position 3 and a pre-soaked disc of Ampicillin for position 4.
12. Incubate plates overnight at 35 degrees Celsius.
I need a good lab report for these two experiments with a structure of:
Objectives:
Introduction:
Materials and Methods:
Results:
Discussion/Conclusion:
I can write up the results part. Many thanks in advance.
Experiment 1: Effect of organic challenge on the efficacy of Milton fluid.
This experiment is designed to demonstrate two concepts. Firstly, that a disinfectant, like Milton Fluid, will kill microbial contaminants on surfaces such as glass. Secondly, to evaluate the effect of organic challenge on the ability of Milton to act as a disinfectant. Milton is 1% Sodium hypochlorite containing 16.5% Sodium Chloride.
Method
i. Label 3 universal bottles A, B and C.
ii. Pour milk into universals A and B until they are 1/3 full.
iii. Let stand for 5 mins.
iv. Pour milk down sink.
v. Clean universal A, but not B with detergent and bottle brush.
vi. Add Milton solution to all universals (A, B and C).
vii. Let stand for 15 mins.
viii. Pour Milton solution down the sink.
ix. Using aseptic technique, divide the nutrient broth provided between the 3 universals A, B and C.
x. Incubate overnight at 35 degrees Celsius.
Experiment 2: Antibiotic disk test (Perform antibiotics test assay).
1. Three test stains are provided with this test 1) E.coli, 2) Staphylococcus "albus" (epidermidis), 3) Bacillus subtilis; antibiotics are unless announced otherwise provided a) Penicillin, b) Cefoxitin, c) Ampicillin. Antibiotics b) and c) are provided as pre-soaked discs while a) is provided as 100mg/1 and 10mg/1 solution.
2. Open sterile swab without touching the cotton tip.
3. Open source culture using aseptic technique.
4. Dip cotton, tip of swab into broth.
5. Carefully 'paint' the entire surface of a sterile agar plate with the cotton bud to cover all areas of the plate. (This will grow up to become a lawn of bacteria.)
6. Label the bottom of the plate with the numbers 1 to 4 evenly spaced out around the table.
7. Sterilize a forceps using alcohol. Danger - check the location the fire blanket in the lab. Keep the alcohol at least 20cm away from Bunsen. If the alcohol container catches fire, cover with a lid and call a demonstrator.
8. Dip a sterile disc into the Penicillin 10mg/1 dilution, shake off excess.
9. Place this carefully onto the surface of the plate at the position marked 1.
10. Repeat for the 100mg/1 Penicillium solution, putting the disc at location 2.
11. Use a pre-soaked disc Cefoxitin. For position 3 and a pre-soaked disc of Ampicillin for position 4.
12. Incubate plates overnight at 35 degrees Celsius.
I need a good lab report for these two experiments with a structure of:
Objectives:
Introduction:
Materials and Methods:
Results:
Discussion/Conclusion:
I can write up the results part. Many thanks in advance.
