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11 Apr 2019
The ASH1 protein is part of a histone deacetylase complex that plays important roles in cell cycle progression in yeast. ASH1 mRNA is known to be transported to daughter cells before cytokinesis, and was one of the first known examples of mRNA localization. Propose a set of experiments that would enable you to determine:
a. What portion of the ASH1 mRNA is the localization sequence (i.e., is necessary to traffic ASH1 mRNA to its destination).
b. What proteins ASH1 interacts with that may be involved in trafficking it
In your responses, note what genetic constructs/manipulations you would make to help you address the question at hand.
The ASH1 protein is part of a histone deacetylase complex that plays important roles in cell cycle progression in yeast. ASH1 mRNA is known to be transported to daughter cells before cytokinesis, and was one of the first known examples of mRNA localization. Propose a set of experiments that would enable you to determine:
a. What portion of the ASH1 mRNA is the localization sequence (i.e., is necessary to traffic ASH1 mRNA to its destination).
b. What proteins ASH1 interacts with that may be involved in trafficking it
In your responses, note what genetic constructs/manipulations you would make to help you address the question at hand.
bladeclassLv1
3 Feb 2023
Reid WolffLv2
14 Apr 2019
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