Question

1. Which of the following statements is NOT true of the behavior of proteins in SDS
PAGE?
a. The rate of movement of the proteins depends on their charge density
b. The rate of movement of the proteins depends on their shape
c. The rate of movement of the proteins depends on their isoelectric point
d. The rate of movement of the proteins depends on their mass
e. All of the above is true

2. Suppose you ran an ion-exchange chromatography and discovered that three proteins
were all bound by the column. Assuming that this is the only column that you have, what
would be the best strategy to get the samples to elute as distinct peaks?
a. Change the pH of the elution buffer to a new value
b. Apply a gradient of pH
c. Apply a gradient of Salt
d. All of the above
e. b and c