Question

PCR was used to amplify a small region at the 5’ end of the coding region of a mutant allele in yeast. This region was sequenced, with the predicted RNA transcript given below and grouped in codons:

Wild-type 5’ GAA CUC GAG CUA AAU 3’

Mutant 5’ GAA CUC GAG CUA AUU 3’

Which of the following are applicable to this mutation (check coding dictionary):

a) transition and nonsense

b) transversion and missense

c) transversion and nonsense

d) transition and missense

e) frameshift