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2 Aug 2019

Which of the following reagents and biomolecules are necessary to make a recombinant DNA ?

a. Restriction enzyme e. Chymotrypsin

b. DNA fragment to be cloned f. Calcium chloride

c. Glucose g. starch

d. DNA ligase h. Ampicillin

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Related questions

Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process?

a) DNA polymerase

b) RNA polymerase

c) A restriction enzyme

d) DNA ligase

e) An aminoacyl-tRNA synthetase

f) Peptidyl transferase

g) Reverse transcriptase

How are restriction enzymes used to insert foreign DNA into plasmids?

a. use the same restrcition enzyme to cut the insert and the vector, then ligate them together with DNA ligase

b. use a restriction enzyme to cut the insert into several fragments, then allow the fragments to recombine with the vector

c. use a restriction enzyme to cut the vector into several fragments, then use DNA ligase to reassemble the fragments around the insert.

d. use a unique restriction enzyme to cut the insert and the vector, then use Taq polymerase to extend the sticky ends, followed by treatment with DNA ligase

e. none of these procedures will work

Recombinant DNA Technology

The questions all relate to the same topic and are somewhat interrelated

1. For gene cloning, a geneticist digests DNA with ___ an enzyme that cleaves DNA at sequence-specific sites.

A. DNA polymerase.

B. Ligase.

C. Restriction endonuclease.

D. cDNA.

2. Certain endonucleases cut DNA and leave DNA termini without overhangs which are called ___

A. Cohesive termini.

B. Sticky ends.

C. Blunt ends.

D. Oligonucleotides.

3. An enzyme found in retroviruses (like HIV) that can be used to prepare DNA fragments from mRNA is called ___.

A. Reverse transcriptase.

B. RNA polymerase.

C. DNA polymerase.

D. Integrase.

4. After combining DNA fragments in a cloning experiment, ___ is used to covalently to join the DNA segments.

A. Restriction endonuclease.

B. DNA ligase.

C. DNA polymerase.

D. Helicase.

a. Fred and Helen’s conceived child: Child ____

b. George and Helen’s conceived child: Child ____

c. George and Helen’s adopted child: Child ____

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