3. You also speculate that various transcription factors could be regulating Tral expression. One such factor is Snail.
(a) If Snail is a transcription factor activating Tral expression, which of the following could potentially be a function of Snail? Select the best answer.
A. Inhibit the binding of RNA polymerase at the Tral Promoter
B. Recruit a histone-modifying protein that opens up the chromatin at the Tral gene.
C. Recruit DNA helicase at the origin of replication.
(b) You want to determine whether Snail binds to the Tral promoter DNA. Which technique(s) would allow you to determine whether this occurs. Select all that apply.
A. Quantitative RT PCR
B. Chromatin Immunoprecipitation
C. Immunoprecipitation
D. Western blot
E. DNA footprinting
4. Now you want to determine the effect of the loss of Snail of Tral -GFP expression. If Snail is an activator of transcription, draw/describe the expected results from a:
A. Northern Blot
B. Q-RT PCR reaction
3. You also speculate that various transcription factors could be regulating Tral expression. One such factor is Snail.
(a) If Snail is a transcription factor activating Tral expression, which of the following could potentially be a function of Snail? Select the best answer.
A. Inhibit the binding of RNA polymerase at the Tral Promoter
B. Recruit a histone-modifying protein that opens up the chromatin at the Tral gene.
C. Recruit DNA helicase at the origin of replication.
(b) You want to determine whether Snail binds to the Tral promoter DNA. Which technique(s) would allow you to determine whether this occurs. Select all that apply.
A. Quantitative RT PCR
B. Chromatin Immunoprecipitation
C. Immunoprecipitation
D. Western blot
E. DNA footprinting
4. Now you want to determine the effect of the loss of Snail of Tral -GFP expression. If Snail is an activator of transcription, draw/describe the expected results from a:
A. Northern Blot
B. Q-RT PCR reaction
Related textbook solutions
Related questions
1) You would like to determine the lacZ expression in thepresence and absence of lactose containing media in E.coli. Which of the following experimental techniques willallow you to do so?
| a) | Fluorescence microscopy of cells expressing GFP taggedlacZ. |
| b) Southern blot |
| c) | Northern blot |
| d) | PCR |
| e) | DNA footprinting |
| f) | Fluorescence hybridization of the lacZ gene |
| g) | Western blot |
| h) | RT-PCR |
Multiple choices are possible
