Question

I am performing a gel extraction to purify DNA after a doubledigest with EcoRI and BamHI. After the gel extraction I need tocomplete a ligation step before bacterial transformation. Theproblem I am having is that the DNA after the gel extraction havesome kind of chemical contaminant that won't allow me to quantifythe DNA using the Nanodrop spectrophotometer. I am getting very low260/230 ratios.

Has anyone had some issues with this?

I am using the Qiagen Qiaquick Gel Extraction Kit.