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14 Sep 2018
What would the most likely phenotype of an E. coli colony containing pUC18 (without an insert fragment) be if the following de novo mutations occurred early in the growth of a single cell into a colony on your plate (assuming plate contains X-gal, IPTG and Amp)? a. A frameshift mutation in the fifth codon lacZ gene in pUC18. b. A loss of the pUC18 plasmid. c. A deletion of bps at positions 19-25 of the ampicillin resistance gene open reading frame.
What would the most likely phenotype of an E. coli colony containing pUC18 (without an insert fragment) be if the following de novo mutations occurred early in the growth of a single cell into a colony on your plate (assuming plate contains X-gal, IPTG and Amp)? a. A frameshift mutation in the fifth codon lacZ gene in pUC18. b. A loss of the pUC18 plasmid. c. A deletion of bps at positions 19-25 of the ampicillin resistance gene open reading frame.
Bunny GreenfelderLv2
15 Sep 2018
