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31 Mar 2018

Give a detailed introduction of Dark Field Microscopy and it's significance to cell biology.

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Lelia Lubowitz
Lelia LubowitzLv2
2 Apr 2018
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Related questions

LAB MODULE: BASIC MICROSCOPY – STUDY HELP

The information in this document is helpful in preparing for the Basic Microscopy Pre-Lab Quiz on Canvas and the Basic Microscopy quiz on Late Nite Labs.

Instructions:

• Go to your Late Nite Labs course site and click on the Basic Microscopy link. The lab manual content will appear on the right side of the screen.

• PRINT and READ all of the information found in the Basic Microscopy lab manual.

• Use the information to answer the following questions.

Questions

• Fill in the remainder of the chart.

Objective Lens

Objective Magnification

Ocular Magnification

Total Magnification

Scanning

4X

10X

40X

Low Power

10X

10X

High Power

40X

10X

Oil Immersion

100X

10X

• Do all organisms consist of more than one cell?

• What is the structural and functional unit of living things? In other words, what is considered the basic building block of LIFE?

• What three structures are found in the cells of ALL organisms, both prokaryotic and eukaryotic? You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all three structures. Note: plasma membrane = cell membrane

• List the major differences in prokaryotic cells and eukaryotic cells? Include the location of genetic material in a eukaryotic versus prokaryotic cell.

• List the organelles found in a eukaryotic cell that are not found in a prokaryotic cell. You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all of these.

• In eukaryotic cells, genetic information is found in the form of DNA. Is this true for prokaryotic cells?

• Are flagella and cell walls unique to eukaryotes? Why or why not?

• The __________is the part of a cell that allows the movement of substances into and out of the cell and protects the cell from its environment.

• The__________is a substance that contains all of the dissolved and suspended ions and other molecules needed to keep the cell alive.

• A__________is an organelle that serves as the site of photosynthesis in a eukaryotic cell.

• Some organisms have cells that contain a__________that gives the cell a more structured shape and strengthens the cell by osmotic pressure.

• Describe dark field microscopy.

• Describe phase contrast microscopy.

• Why do scientists use dark field microscopy and phase contrast microscopy when studying prokaryotic cells?

• How does phase contrast microscopy help scientists visualize difficult specimens?

• Fill in the remainder of the chart. The organisms studied during this lab are: Amoeba, Spirogyra, cardiac muscle, and bacteria.

Structure Function Organisms Studied
Plasma Membrane

Amoeba, Spirogyra, cardiac muscle, and bacteria
Cytoplasm
Nucleus

Contains the genetic material of a eukaryotic cell
Chloroplast

Spirogyra
Cell wall

Q: Compare and contrast the different types of microscopy?

Q: For each of the following types of light microscopes briefly describe it; state some specific uses; state some limitations of use (if applicable):

(a) Bright field

(b) Dark field

(c) Phase Contrast

(d) Fluorescent

(e) SEM

(f) TEM

Please help. Thanks!

Epifluorescence microscopy techniques have revolutionized modern cell and molecular biology. A) You have identified a new protein and you want to know where in the cell the protein is localized, as well as whether the protein interacts with other known signaling molecules. How would you use fluorescence microscopy to show the subcellular location of the protein? What reagents would you need? B) You suspect the protein is a motor protein that interacts with microtubules. How would you determine if this hypothesis is correct using microscopy?

Please answer every part. Thank you.

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