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31 Jan 2018
1.
a.) A tissue culture flask containing cells is cultured in 10mL of media containing 0.01g/mL glucose. If the cells consume glucose at a rate of 0.01 g/hour, how long before the glucose level in the media reaches 0.001g/mL? Why does this model not accurately represent the rate at which nutrients are consumed by cells?
b.) If these cells are grown in a 10mL bioreactor which flows fresh media (containing 0.01g/mL glucose) into the system and continually ejects an equal volume of the existing media, what flow rate must be used to maintain a 0.005g/mL glucose level in the system? Assume a well-mixed system.
1.
a.) A tissue culture flask containing cells is cultured in 10mL of media containing 0.01g/mL glucose. If the cells consume glucose at a rate of 0.01 g/hour, how long before the glucose level in the media reaches 0.001g/mL? Why does this model not accurately represent the rate at which nutrients are consumed by cells?
b.) If these cells are grown in a 10mL bioreactor which flows fresh media (containing 0.01g/mL glucose) into the system and continually ejects an equal volume of the existing media, what flow rate must be used to maintain a 0.005g/mL glucose level in the system? Assume a well-mixed system.
Nelly StrackeLv2
2 Feb 2018
