A mixture of three proteins (A,B,C) can be thereetically separated by anion exchange chromotgraphy using cellulose coated with diethylamino-ethyl groups (pka 10.5). In the first step the mixture was applied to the resin at pH 7.5 and washed with the same buffer to elute protein(s) that did not bind. The resin was then washed with a buffer at pH 4.5
Protein A IS A HOMODIMER WITH A MOLECULAR WEIGHT OF 50 kda AND A pi OF 3.0 PROTEIN b IS A HETEROTETRAMER WITH A MOLECULAR WEIGHT OF 100 Kda AND A pi OF 6.0 PROTEIN C IS A MONOMER WITH A MOLECULAR WEIGHT OF 75 Kda AND A pI OF 9.0
WHICH OF THE FOLLOWING STATEMENTS IS INCORRECT?
A. PROTEINS A AND B ARE INITIALLY BOUND TO THE RESIN AT PH 7.5
B. THE RESIN ITSELF WILL REMAIN POSTITIVELY CHARGED OVER THE PH RANGE 3 TO 7.5
C. ONLY PROTEIN A WILL ELUTE WHEN THE RESIN IS WASHED WITH A BUFFER AT PH 4.5
D. PROTEIN C DOES NOT BIND TO THE RESIN AT PH 7.5
A mixture of three proteins (A,B,C) can be thereetically separated by anion exchange chromotgraphy using cellulose coated with diethylamino-ethyl groups (pka 10.5). In the first step the mixture was applied to the resin at pH 7.5 and washed with the same buffer to elute protein(s) that did not bind. The resin was then washed with a buffer at pH 4.5
Protein A IS A HOMODIMER WITH A MOLECULAR WEIGHT OF 50 kda AND A pi OF 3.0 PROTEIN b IS A HETEROTETRAMER WITH A MOLECULAR WEIGHT OF 100 Kda AND A pi OF 6.0 PROTEIN C IS A MONOMER WITH A MOLECULAR WEIGHT OF 75 Kda AND A pI OF 9.0
WHICH OF THE FOLLOWING STATEMENTS IS INCORRECT?
A. PROTEINS A AND B ARE INITIALLY BOUND TO THE RESIN AT PH 7.5
B. THE RESIN ITSELF WILL REMAIN POSTITIVELY CHARGED OVER THE PH RANGE 3 TO 7.5
C. ONLY PROTEIN A WILL ELUTE WHEN THE RESIN IS WASHED WITH A BUFFER AT PH 4.5
D. PROTEIN C DOES NOT BIND TO THE RESIN AT PH 7.5
