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26 Jul 2019

Calculate the following solutions and explain how you would prepare them.

1) 400 ml of 60% (v/v) SDS

2) 150 ml of 2.5 M Tris (Trisaminomethane) (MW = 121.2)

3) 900 ml of 50% EDTA (w/v)

4) 25 ml of 0.5 M NaOH

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Jarrod Robel
Jarrod RobelLv2
28 Jul 2019

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Related questions

3) You have the following stock solutions and solid chemicals in your lab:

-2.0 M NaCl

-0.50 M Tris buffer, pH 8.0

-20 mg/ml Bovine serum albumin (BSA)

--MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95 % pure)

-Glucose (solid, anhydrous)

How would you make 250 ml of a solution containing all of the following:

0.1M NaCl,

0.02 M Tris, pH 8.0,

0.5 mg/ml BSA,

25 mM MgCl2,

150 mg% glucose.

List the volumes of each stock solution and the weights of each solid. Include calculations for each component.

3. Write the calculations to make the following solutions using the stocks and solids listed above. Assume all the above solutions are in deionized water.

a. How would you prepare a 500 mL solution containing 1.5 M Tris and 20% KCl?

b. How would you prepare a 0.75 L solution containing 50 mM NaCl and 10% SDS?

c. How would you prepare a 100 mL solution containing 1% agarose and 1x TBE?

d. How would you prepare a 250 mL solution containing 250 mM Tris, 5 g/L NaCl, and 50% SDS?

E. How would you prepare a 200 μL solution containing 1% glycerol?

A. You are given 50 µL of a highly concentrated DNA solution. You need to prepare 1.0 mL of a 1:500 dilutions. Describe how you would prepare this solution

B. You diluted a DNA solution 1:100 and measured the OD at 260 nm. The spectrophotometer gave you a reading of 0.35. Describe how would you determine the DNA concentration of your original solution? Remember, the εDNA = 50x10-3 mg/mL.

You prepare a solution by adding the following ingredients inthe order indicated: 600 mL H2 O, 125 mL 1.6 M LiCl, 50 mL 20 %(m/v) MgCl2 , and 25 mL 10g/L NaCl. The properties of eachingredient are as follows: LiCl: MW 200g/mole, density 1.2g/mL,density of 1.6 M soln. 1.05g/mL MgCl2: MW 150g/mole, density1.3g/mL, density of 20% soln. 1.1g/mL NaCl: MW 35g/mole, density1.15g/mL, density of 10g/L soln. 1.03g/mL Final solution: density:1.25g/mL

1. What is the final molarity of MgCl2 in the solution?

The answer is 0.083. I just don't know how to get thatanswer

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