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19 Jun 2019

Give a description of the process of conjugation.

How could one get an Hfr strain of bacteria?

What is the most likely result of a mating between an Hfr strain x F– strain and why?

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Reid Wolff
Reid WolffLv2
20 Jun 2019

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Related questions

) An Hfr strain (arg+, bio+, leu+) is mated to an F- strain (arg-, bio-, leu-). Assume that this Hfr strain transfers arg+ last. You grow the exconjugants on arg- media, supplemented with leu+ and bio+. You recover 423 arg+ exconjugants. These are tested individually for bio and leu and you find the following distribution of genotypes:

arg+, bio+, leu+ 360

arg+, bio+, leu- 9

arg+, bio-, leu+

0 arg+, bio-, leu- 54

a) What is the gene order (show your reasoning)? b) What are the frequencies of recombination between each pair of genes?

What is the difference between a selected marker and a counterselected marker? Why are both necessary in an Hfr X F- mating?
A conjugation experiment is performed to map the location of three genes involved in the ability to synthesize three amino acids, histidine (His), tryptophan (Trp), and leucine (Leu). An Hfr strain with genotype met-his+leu+trp+ is mated with a met+his-leu-trp- recipient. The met marker, which (confers the ability to synthesize the amino acid methionine) is known to be transferred very late. After a short mating the cells are broken apart and plated on four different growth media. The amino acids present in the growth medium and the number of colonies observed on each are the following: 
His, Trp: 250 
His, Leu: 50 
Leu, Trp: 500 
His only: 10 
A) What is the order of injection of the genes? 
Please provide explanation.

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