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17 May 2018
DNA PURIFICATION QUESTIONS:
Why is it important to remove as much as possible of the growth medium from the cell pellet during cell harvesting?
Alice and Frankie are lab partners in a micro lab performing DNA extraction, each person using a different bacterial species. Both of them spun 1.8 ml of their overnight broth cultures in a microcentrifuge, and it turned out that Aliceâs tube had a substantially larger pellet than Frankieâs. What could Frankie do to increase the amount of cell pellet necessary for subsequent extraction steps?
DNA PURIFICATION QUESTIONS:
Why is it important to remove as much as possible of the growth medium from the cell pellet during cell harvesting?
Alice and Frankie are lab partners in a micro lab performing DNA extraction, each person using a different bacterial species. Both of them spun 1.8 ml of their overnight broth cultures in a microcentrifuge, and it turned out that Aliceâs tube had a substantially larger pellet than Frankieâs. What could Frankie do to increase the amount of cell pellet necessary for subsequent extraction steps?
Bunny GreenfelderLv2
19 May 2018
