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17 Apr 2018

Outline in molecular terms how so many isoforms of one glycoprotein [ hSHBG] can occur
together in human serum and Suggest how the chemical differences you have outlined can lead to a range of pI and mass
values..

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Tod Thiel
Tod ThielLv2
19 Apr 2018

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Related questions

I am looking for studies on how many protein isoforms havedifferent functions, preferably in human. We know that a greatmany, if not most, of human genes are alternatively spliced andthat many produce different protein isoforms. Has anyone looked athow many of these isoforms have different cellular functions? Ifsomeone could point me to a published paper, that would begreat.

If no such study has been made, can anyone recommend a databasefrom which this information could be extracted? GeneOntology isgene based so the information cannot be found there. Genes will beannotated to specific terms, not their protein isoforms. Also, Iwould need to be able to do this in a high throughput manner, I amnot interested in specific proteins but in what percentage of allisoforms have different functions.

Ideally, I would like to be able to extract, for every humangene, the list of the different protein isoforms it encodes andwhether their functions differ, or at least what those functionsare.

Protein A 65(kDa) pI (7.2) - glycoprotein

Protein B 72(kDa) pI (7.8) - antibody available; not glycosylated

Protein C 63(kDa) pI (6.8) - not glycosylated

Protein D 70(kDa) pI (8.8) - glycoprotein

Giving the above information on protein components of a medical sample from a human patient that is to be purified :

(a) Propose a suitable series of chromatography steps to separately isolate each of the four protein components of this mixture, whilst maintaining their biological fold. At least one of the series of steps should involve affinity chromatography, present your purification protocol as a flow chart, outlining the types of chromatography you suggest, and the chromatography media required.

(b) Select an affinity step in your procedure. Prepare a full set of instructions for carrying out the step in the protein laboratory. Give chemical names for the chromatography media, buffer system and any salts needed. Include concentrations as well.

ICAM-1 is a glycoprotein found on the outside of human endothelial cells and cells of the immune system. Many different strains of rhinovirus can bind to the ICA-1 receptor via different attachment proteins (ligands). Human produced antibodies regularly target these viral ligand proteins. How come then human antibodies produced in response to one rhinoviral ligand cannot target the ligands of other rhinovirus strains even though these ligands can all bind to ICAM-1?

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