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For the following molecules indicate if the molecule is a DNA/RNA/protein, if it functions in DNA replication/transcription/RNA processing and if it functions in prokaryotes/eukaryotes/both prokaryotes and eukaryotes. Match each letter to the correct protien.

TATA box

poly(A) polymerase

ligase

catalytic component of the splicesome
A.

DNA ; DNA replication; prokaryote
B.

DNA; DNA replication; eukaryote
C.

DNA; DNA replication; prokaryote & eukaryote
D.

DNA; transcription; prokaryote
E.

DNA; transcription; eukaryote
F.

DNA; transcription; prokaryote & eukaryote
G.

RNA; RNA processing; prokaryote
H.

RNA; RNA processing; eukaryote
I.

RNA; RNA processing; prokaryote & eukaryote
J.

RNA; transcription; eukaryote
K.

protein; DNA replication; prokaryotes and eukyarotes
L.

protein; RNA processing; eukaryote
M.

protein; transcription; eukaryote
N.

protein; DNA replication; prokaryote

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Hubert Koch
Hubert KochLv2
29 Sep 2019
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Related questions

Chapter 10

1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcher’s key experiment?

2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?

3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?

4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?

5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?

Gene

Methylation levels

1

++

2

+++++

3

+++

4

++

5

+

What are the characteristics of the 3 main DNA forms?

Chapter 11

What are the different types of chromatin?

What are the structures and important roles for telomeres and centromeres?

What are the differences found between eukaryotic chromosomes and mitochondrial?

Chapter 12

Explain each of the different models of replication.

If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?

Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.

What are the functions of the different DNA polymerases found in eukaryotic cells?

Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)

What is the Holliday model of recombination and what are the necessary steps?

Chapter 13

What are the different types of RNA and what roles do they play?

Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.

Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.

Chapter 14

What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.

What is alternative splicing and what role does it play in the cell?

How is ribosomal RNA processed after transcription?

How do siRNA and miRNA work, describe/draw out the process in detail.

1)How many fragments of DNA will be produced, if you digest pBR322with the restriction enzyme EcoR1?

2) (more than one answer) A mature eukaryotic mRNA containsthe following:
a)Introns
b)Exons
c)Poly A tail
d)A methylated guanine cap
e)UTR our side start and stop codons

3) Which of the following statements are correct ?
a)An eukaryotic promoter contains TATA box
b)A protein molecule terminates with one acidic and one basicends
c) Several transcription factors are involved in initiating agene transcription
d) An RNA primer is needed for gene transcription
e) RNA polymerase || is responsible for eukaryotic genetranscription

Biochem:

1. Indicate whether the following describes DNA polymerase, RNApolymerase, both, or none:

a. Binds to single stranded DNA

b. Uses an RNA primer

c. Has proof-reading ability

d. Reads from 3' to 5'

e. Phosphodiester bonds are formed using rNTPs

f. Requires a DNA primer

g. Multiple types are present in eukaryotes

h. Mutiple types are present in prokaryotes

i. Is synthesized bi-directionally

j. Requires there to be a terminal sequence on DNA

k. Is part of a larger complex made up of several proteins

2. Post-translational damage causes the loss of the 2' hydoxylgroup on several adenosine nucleotides within the intron sequenceof a primary RNA transcript. What could possible happen to theresulting mature RNA sequence and consequent protein?

3. Where on the below strand of RNA would you most likely findthe following:

a. Start of transcription

b. Start of translation

c. TATA box

d. Enhancers

e. Initiator

f. Activators

g. Repressors

4. How would the following mutation in the template strand ofDNA affect the translated protein? (x=any base, / = exon/intronjunction)

5-XXXXC/CTGAAGAGGAAGXXXXGTTAGXXXXACTCAC/CTCGC-3 to5-CCTGAAGAGGAAXXXXXXXXGTTAGXXXXACTCACCTCGC-3

5. When will there be a 5'-5' linkage in RNA? When is this bondformed? Which base is commonly involved in this process? What wouldhappen if there was an inability in this enzyme to make the 5'-5'likage?

6. Consider the following strand of mRNA. What protein would beproduced if this strand was translated?

5'- CGUAUGCCCUUUACUAGUAUGUGAUACUAGUAAACC-3'

7. What would be the new protein if the following mutations weremade to the strand in 6? Label/classify each type of mutation.

a. 5'-CGUAUGCCCUUUAACUAGUAUGUGAUACUAGUUAACC-3'

b. 5'-CGUAUGCCCUUCACUAGUAUGUGAUACUAGUUAACC-3'

c. 5'- CGUAUGCCCUUUACUAGUAUUGAUACUAGUUAACC-3'

d. 5'- CGUAUGCCCUUGACUAGUAUGUGAUACUAGUAAACC-3'

e. 5'-CGUAUGCCCUUCACUAGUAUGAGUAACUAGUAACC-3'

f. 5'-CGUAUGCCCUAAACUAGUAUGUGAUACUAGUAAACC-3'

8. What are examples of two different types of point mutationsin sequences coding for hemoglobin production?

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