A microarray consists of a flat matrix with regularly placed âspotsâ of DNA, each with 107 â 108 copies of the same DNA sequence. (But each spot has a unique DNA sequence.) You can label fragments of human genomic DNA with a fluorescent dye and hybridized the DNA probes to the microarray. If there are sequences present in the genome that can hybridize to the spot, the spot will fluoresce, which can be detected via microscopy. Indeed, you could use such an array to detect base-pair differences among different individuals, so-called SNPs (single nucleotide polymorphisms). How do you imagine such a system would work? (Hint: think about the how the conditions can affect the hybridization of DNA.)
A microarray consists of a flat matrix with regularly placed âspotsâ of DNA, each with 107 â 108 copies of the same DNA sequence. (But each spot has a unique DNA sequence.) You can label fragments of human genomic DNA with a fluorescent dye and hybridized the DNA probes to the microarray. If there are sequences present in the genome that can hybridize to the spot, the spot will fluoresce, which can be detected via microscopy. Indeed, you could use such an array to detect base-pair differences among different individuals, so-called SNPs (single nucleotide polymorphisms). How do you imagine such a system would work? (Hint: think about the how the conditions can affect the hybridization of DNA.)
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Which of the following did Erwin Chargaff observe?
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The melting temperature (Tm) of a DNA duplex is:
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Given your knowledge and the descriptions of each of thesemethods, which of the following does NOT rely on the ability ofnucleic acids to hybridize with each other?
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Which of the following is a possible reason why DNA uses thymineinstead of uracil?
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Which of the following is an enzyme used in cloning to breakcovalent bonds?
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Which of the following could you use to remove the 5' phosphatesafter cleavage of a plasmid with a single type of restrictionenzyme to ensure that the plasmid would not simply fuse backtogether upon addition of ligase?
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Which of the following would you use to create a cDNA librarythat you would not use for a genomic library?
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A plasmid vector typically has which of the followingfeatures?
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Which of the following is FALSE of genomic or cDNAlibraries?
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Which of the following is NOT a typical component of apolymerase chain reaction (PCR)?
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How can a fusion protein be formed?
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Why is green fluorescent protein (GFP) so useful in visualizingfusion proteins in eukaryotes?
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You wish to determine the cellular location of a protein butunfortunately it is not particularly antigenic (can t develop astrong antibody response). How could you solve this problem?
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A positive result for the yeast two-hybrid analysis means thefollowing:
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Which of the following is the approximate size of the humangenome?
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Which of the following is correct about the structure orlocation of genes?
Question 22 options:
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