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1) Which of the following statements is NOT correct?

Select one:

a. In an endospore and an acid-fast stain, heat is used to drive the primary stain into the cells.

b. Botulism is a disease that is caused by an endospore forming bacterial species.

c. Clostridium and Bacillus are two genera that commonly form endospores.

d. The complex of dipicolonic acid and calcium gives the endospores its heat resistant properties.

e. Under optimal growth conditions, vegetative cells are said to be in a cryptobiotic state.

2) ____________________ of cells by transferring the colony or a portion of a colony to a new plate is commonly used by microbiologists to maintain cultures.

Select one:

a. Subculturing

b. Mixing

c. Gram staining

d. Transformation

3) Which of the following statements is correct?

Select one:

a. Crystal violet is considered a differential stain.

b. Sporulation occurs when there are plenty of nutrients and water available.

c. In a gram stain, ethanol is used as the mordant.

d. Clostridium species are capable of undergoing sporulation.

e. Endospores are considered reproductive structure.

4) Which of the following is required for both the Schaeffer-Fulton endospore stain and the Ziehl-Neelson acid-fast stain?

Select one:

a. source of heat

b. alkaline methylene blue

c. ethanol

d. malachite green

e. egg albumin

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Irving Heathcote
Irving HeathcoteLv2
29 Sep 2019
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