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28 Sep 2019
You are given an unknown concentration of cells in a tube and asked to do a serial dilution to obtain a final dilution of 1:10,000. You have 2 additional empty tubes and are asked to make the final volume for each dilution 100 microliters. You will be using phosphate-buffered saline (PBS) to dilute the cells. Explain, in words or diagram, how would you perform this serial dilution to obtain a final dilution of 1:10,000. Make sure you indicate the volume of cells added to each tube, and the volume of PBS in each tube in your answer.
You are given an unknown concentration of cells in a tube and asked to do a serial dilution to obtain a final dilution of 1:10,000. You have 2 additional empty tubes and are asked to make the final volume for each dilution 100 microliters. You will be using phosphate-buffered saline (PBS) to dilute the cells. Explain, in words or diagram, how would you perform this serial dilution to obtain a final dilution of 1:10,000. Make sure you indicate the volume of cells added to each tube, and the volume of PBS in each tube in your answer.
Patrina SchowalterLv2
28 Sep 2019