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1. You are given a culture of a purple-pigmented bacterium. You attempt to culture it under bright lights in a medium containing no reduced carbon source, and it fails to grow. You attempt to culture with glucose in the dark, and it still fails to grow. Last, you culture it with glucose in the light, and the culture grows. This bacterium is behaving as a:

A: Facultative Chemoheterotroph

B: Obligate Chemoheterotroph

C: Obligate Photoheterotroph

D: Facultative Photoautotroph

2. Organisms defined as autotrophs can:

A: Only use reduced carbon sources for biosynthesis

B: Only use the power of light to produce energy

C: Take CO2 and convert it to organic molecules for biosynthesis

D: Only survive if they are in an atmosphere devoid of oxygen

3. Organisms defined as heterotrophs can:

A: Use reduced carbon sources for biosynthesis

B: Only use the power of light to produce ATP

C: Take CO2 and convert it to organic molecules for biosynthesis

D: Only survive if they are in an atmosphere without oxygen

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Trinidad Tremblay
Trinidad TremblayLv2
28 Sep 2019
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Related questions

Multiple Choice

1. Which of the following describes lipids?

a source of nutrients for organisms 


energy-storage molecules 


molecules having structural role in membranes 


molecules that are part of hormones and pigments 


all of the above 


2. Molecules bearing both polar and nonpolar groups are said to be which of the following?

a. hydrophilic


b. amphipathic

c. hydrophobic

d. polyfunctional

3. By definition, carbohydrates contain which elements?

carbon and hydrogen 


carbon, hydrogen, and nitrogen 


carbon, hydrogen, and oxygen 


carbon and oxygen 


4. Monosaccharides may link together to form polysaccharides by forming which type of bond?

a. hydrogen

b. peptide


c. ionic


d. glycosidic

5. Which of the following is the name for molecules whose structures are nonsuperimposable mirror images?

structural isomers 


monomers 


polymers 


enantiomers 


Which of the following is an organism that obtains its energy from the transfer of electrons originating from chemical compounds and its carbon from an inorganic source?

a. chemoautotroph

b. chemoheterotroph

c. photoheterotroph

d. photoautotroph

7. A cell might perform anaerobic respiration for which of the following reasons?

It lacks glucose for degradation. 


It lacks the transition reaction to convert 
pyruvate to acetyl-CoA. 


It lacks Krebs cycle enzymes for processing 
acetyl-CoA to CO2. 


It lacks a cytochrome oxidase for passing 
electrons to oxygen. 


8. Which of the following is not an electron carrier within an electron transport system?

a. flavoprotein

b. ATP synthase

c. ubiquinone

d. cytochrome oxidase

9. Which of the following is the purpose of fermentation?

a. to make ATP

b. to make carbon molecule intermediates for anabolism

c. to make NADH

d. to make NAD+

10. Which of the following processes produces hydrogen sulfide?

anoxygenic photosynthesis 


oxygenic photosynthesis 


anaerobic respiration 


chemoautrophy 


11. In which growth phase would you observe the most endospores in a Bacillus cell culture?

death phase 


lag phase 


log phase 


log, lag, and death phases would all have roughly 
the same number of endospores. 


12. Which of the following is the best definition of generation time in a bacterium?

a. the length of time it takes to reach the log phase 


b. the length of time it takes for a population of 
cells to double 


c. the time it takes to reach stationary phase 


the length of time of the exponential phase 


If a culture starts with 50 cells, how many cells will be present after five generations with no cell death?

a. 200

b. 400

c. 1600

d. 3200

14. During which phase would penicillin, an antibiotic that inhibits cell-wall synthesis, be most effective?

death phase 


lag phase 


log phase 


stationary phase 


15. Pseudomonas aeruginosa is a common pathogen that infects the airways of patients with cystic fibrosis. It does not grow in the absence of oxygen. The bacterium is probably which of the following?

an aerotolerant anaerobe 


an obligate aerobe 


an obligate anaerobe 


a facultative anaerobe 


16. Which of the following refers to photosynthesis performed by bacteria with the use of water as the donor of electrons?

a. oxygenic


b. anoxygenic

c. heterotrophic

d. phototrophic

17. Which term best describes the relationship between pathogenic bacteria and humans?
A. amensalism

B. commensalism

C. parasitism

D. predation

18. Which describes bacteria that require low concentrations of oxygen to survive (less than that in the current atmosphere)?

A. facultative anaerobes

B. microaerophiles

C. obligate aerobes

D. obligate anaerobes

19. Cyanobacteria produce which of the following?

A. hydrogen sulfide gas
B. large quantities of ammonia

C. oxygen*
D. sulfuric acid

20.How do Archaea differ from Bacteria?

A. Archaea are found exclusively in extreme habitats.
B. Archaea have ether linkages in their cell membranes.*

C. Archaea have peptidoglycan cell walls.

D. Archaea lack a nucleus.

Microbiology Unit 2 Applied and Environmental Assignment Part 1: Identify Nutritional requirements from Chapter 6&14 (and review from chapters 1 & 2) 1. Determine which of the 4 macromolecules require the following elements: a. Carbon: carbohydrates___ lipids___ proteins___ nucleic acids___ b. Nitrogen: carbohydrates___ lipids___ proteins___ nucleic acids___ c. Sulfur: carbohydrates___ lipids___ proteins___ nucleic acids___ 2. The essential elements mentioned above are cycled through cells and the earth in biogeochemical cycles (the carbon, nitrogen, and sulfur cycles.)Select the correct answer or fill in the blanks to summarize these cycles: a. Carbon dioxide is taken in from the atmosphere by [autotrophs/heterotrophs] and converted to glucose through carbon ________________. When heterotrophs eat autotrophs they use catabolism to break down glucose to make ATP and waste carbon is released as ___________. b. There is plenty of nitrogen available in the atmosphere but it is not accessible to organisms because it is in the form of Nitrogen gas (N2). N2 is fixed by [bacteria/plants/animals] and converted to ammonia (NH3.) NH3 can be used by [bacteria/plants/animals] to build their cells. Next, by consuming food with amino acids,[bacteria/plants/animals] can get access to nitrogen for their own cellular needs. c. There is also a good quantity of sulfur in dirt, rocks, and the atmosphere. Sulfur cycles between H2S => S0 => SO4 => SH groups in Proteins => back to H2S.Using _________________ respiration, anoxygenic photosynthesis, and chemolithoautotrophy [bacteria/plants/animals]convert H2S and S0 to SO4 which is taken in by [bacteria/plants/animals] and used to build their cells. When [bacteria/plants/animals]eat plants they take in essential amino acids containing sulfur and recycle these to make their proteins. d. _________________ break down waste and dead organisms and recycle nutrients back into the cycles. *The C and N are summarized here: https://youtu.be/2D7hZpIYlCA and https://youtu.be/leHy-Y_8nRs 3. Match the main metabolic classifications to the description: Classifications: photoautotroph/ photoheterotroph/ chemoautotroph/ chemoheterotroph o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from sunlight o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from reduced inorganic chemicals o A microbe that eats other organisms to get organic carbon and energy o A microbe that eats other organisms to get organic carbon but gets energy from sunlight 4. Identify the phases of the bacterial growth curve and define what occurs in each phase Phase A = ______________ Definition: Phase B = ______________ Definition: Phase C = ______________ Definition: Phase D = ______________ Definition: Part 2: Unique ecosystems containing microbes 5. Watch the video that shows hydrothermal vents: https://youtu.be/RO3nO98gH_M a. Describe bacteria that live at the vents using terms from the unit 2 reading guide: ________________________________________________________________________ b. Write a 2-3 sentence reflection about hydrothermal vents and the contribution of microbes to this ecosystem: 6. Apply what you have learned to the TED-Ed video about Biofilms: https://youtu.be/pHLP5CZMnL4 a. Give the terms for cell structures mentioned in the video that we learned in Chapter 4 and 7: i. “sticky appendages” thatallows bacteria to attach and begin the biofilm: _______________ ii. Extracellular matrix/gooey matrix that holds cells together: ____________________________ iii. Exchange genetic information while packed together: ____________________________ iv. Communicate with each other by _________________________________________ sensing. v. Produce chemical to kill microbial competitors: ____________________________ factor.

Question 1

A microorganism's ability to use a harmful pesticide as a source of carbon and energy is an example of

Select one:

a. chemoautotrophy

b. nitrogen fixation

c. bioremediation

d. photoautotrophy

e. biosynthesis

Question 2

Autotrophs and heterotrophs differ in

Select one:

a. their ability to utilize CO2 as a sole carbon source.

b. their ability to incorporate CO2 into cells.

c. only a few enzymes.

d. their ability to synthesize precursor molecules.

e. their ability to utilize CO2 as a sole energy source.

Question 3

If the amino acid valine constituted approximately 4% of the total cell protein of a bacterium, how much valine should be added to a defined medium in order to grow 1 gram of this organism?

Select one:

a. 0.2 mg

b. 2 mg

c. 20 mg

d. 40 mg

e. 200 mg

Question 4

Transport of HPO4 along with a proton (H+) is an example of

Select one:

a. antiport

b. uniport

c. facilitated diffusion

d. symport

e. group translocation

Question 5

The energy required for transport via ABC transporters is derived from

Select one:

a. a proton gradient

b. an electrical charge separation across the membrane

c. ATP hydrolysis

d. a symport mechanism

e. a high energy phosphate bond of PEP

Question 6

The spread plate method would clearly be preferred over the pour plate method when isolating colonies of

Select one:

a. anaerobic bacteria

b. thermophilic bacteria

c. bacteria from temperate lake zones

d. purple non-sulfur bacteria

e. agar-degrading bacteria

Question 7

Enrichment culture procedures for many microorganisms require intial growth in liquid media, followed by growth on solid media. The solid media is used to

Select one:

a. provide proper growth conditions.

b. isolate single colonies of the microbes.

c. get rid of competing microorganisms.

d. isolate strict aerobes.

e. enrich for specific nutrients.

Question 8

The optical density of light scattered by bacteria is a very useful tool for estimating population size. What can alter the bacteria's light-scattering properties?

Select one:

a. Cell volume depending on its growth stage

b. The condition of being absent of microorganisms.

c. The amount of light projected from the room.

d. The standard curve

e. Standard deviation from growth curve.

Question 9

What is true about a bacterial colony?

Select one:

a. It arises from an individual cell and represents an isolate strain.

b. Colonies, and CFU (colony forming units) on streak plates represent a diverse microbial culture.

c. In order to obtain a colony, on must perform cloning steps.

d. Axenic cultures to not represent CFUs or colonies.

e. Colonies require differential media in order to separate them from the population.

Question 10

What is a biofilm?

Select one:

a. All cells in a population achieve a steady state

b. A specialized, surface-attached, collaborative community of bacteria

c. A complex media made of the chemical building blocks that a cell needs

d. A type of anaerobic growth chamber.

e. A type of spore

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