A student written question: New Belgium Brewery creates its own yeast strains for brewing beer. Some yeast strains secret a toxic protein, which is not detectable until after the brewing process. Therefore the brewery must find a way to detect the yeast that secretes this toxic protein. Because they collaborate with Dr. Wolkow, a yeast-whiz, the brewery decides to hire you to work with Dr. Wolkow to make a cDNA expression library from the toxin-secreting strain. You decide to use a bacterial expression vector since it is easy to work with.
1. What components must the expression vector contain?
a. promoter, translation start sequence, cloned cDNA sequence, DNA polymerase
b. DNA ligase, translation start signal, cloned cDNA sequence, polyA tail
c. Shine-Dalgarno sequence, promoter, cloned cDNA sequence, antibody
d. promoter, Shine-Dalgarno sequence, cloned cDNA sequence, transcription termination sequence
2. Suppose the radioactive 3 antibody did not attach to any of the protein products. What can you conclude from this?
a. the toxic protein product is produced by all the yeast strains
b. the toxic protein product is produced by one or more of the yeast strains
c. the toxic protein product is not produced by any of the yeast
d. the yeast DNA was destroyed when the cells were lysed
3. Why would the brewer want to specifically use an expression vector rather than another type of vector?
a. It is specifically for eukaryotic DNA
b. It is used to produce the protein encoded by a cloned gene
c. It is only used for cloning cDNA sequences
d. They want to produce mRNA encoded by a cloned gene
4. This type of blot, where protein is transferred onto the membrane and is then hybridized to a tagged-antibody is a blot.
a. Southern
b. Northern
c. western
d. southwestern
A student written question: New Belgium Brewery creates its own yeast strains for brewing beer. Some yeast strains secret a toxic protein, which is not detectable until after the brewing process. Therefore the brewery must find a way to detect the yeast that secretes this toxic protein. Because they collaborate with Dr. Wolkow, a yeast-whiz, the brewery decides to hire you to work with Dr. Wolkow to make a cDNA expression library from the toxin-secreting strain. You decide to use a bacterial expression vector since it is easy to work with.
1. What components must the expression vector contain?
a. promoter, translation start sequence, cloned cDNA sequence, DNA polymerase
b. DNA ligase, translation start signal, cloned cDNA sequence, polyA tail
c. Shine-Dalgarno sequence, promoter, cloned cDNA sequence, antibody
d. promoter, Shine-Dalgarno sequence, cloned cDNA sequence, transcription termination sequence
2. Suppose the radioactive 3 antibody did not attach to any of the protein products. What can you conclude from this?
a. the toxic protein product is produced by all the yeast strains
b. the toxic protein product is produced by one or more of the yeast strains
c. the toxic protein product is not produced by any of the yeast
d. the yeast DNA was destroyed when the cells were lysed
3. Why would the brewer want to specifically use an expression vector rather than another type of vector?
a. It is specifically for eukaryotic DNA
b. It is used to produce the protein encoded by a cloned gene
c. It is only used for cloning cDNA sequences
d. They want to produce mRNA encoded by a cloned gene
4. This type of blot, where protein is transferred onto the membrane and is then hybridized to a tagged-antibody is a blot.
a. Southern
b. Northern
c. western
d. southwestern
