DNA is isolated from person X and is seperately digested with restriction enzymes EcoRI and Hhal. What is the expected occurrence of restriction sites for Hhal? Which enzyme is expected to produce the lower % of smaller restriction fragments? Which enzyme is expected to have restriction sites at the lower frequency in the human genome?
DNA is isolated from person X and is seperately digested with restriction enzymes EcoRI and Hhal. What is the expected occurrence of restriction sites for Hhal? Which enzyme is expected to produce the lower % of smaller restriction fragments? Which enzyme is expected to have restriction sites at the lower frequency in the human genome?
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The following table presents the results of a restriction enzyme cutting analysis with different enzymes for a given DNA sequence.
| Enzyme | Number of sites | Position of Sites | Recognition Sequence |
| AccB7I | 2 | 268 380 | ccannnn/ntgg |
| AcsI | 1 | 548 | r/aatty |
| AfeI | 1 | 706 | agc/gct |
| Alw21I | 5 | 90 584 756 972 1017 | gwgcw/c |
| Ama87I | 1 | 410 | c/ycgrg |
| AocI | 2 | 662 796 | cc/tnagg |
To produce the maximal number of small DNA fragments, which enzyme should be chosen?
We have four DNA sequences that we need to digest (cut up), and have 6 restriction enzymes in stock in our lab. We want to find out which restriction enzyme we can use on each sample.
For each sequence, please indicate which restriction enzyme(s) would cut within each sequence. Each sequence may be cut by one, more than one, or no restriction enzymes.
Following the sequences are two questions. Please choose from the same list of enzymes to indicate the correct answer to these questions.
| Restriction enzyme | Digestion pattern |
| EcoRI | 5' G|AATTC 3' 3' CTTAA|G 5' |
| HindIII | 5' A|AGCTT 3' 3' TTCGA|A 5' |
| BamHI | 5' G|GATCC 3' 3' CCTAG|G 5 |
| AluI | 5' AG|CT 3' 3' TC|GA 5' |
| SmaI | 5' CCC|GGG 3' 3' GGG|CCC 5' |
| HbaI | 5' GCG|C 3' 3' C|GCG 5' |
(The | symbol indicates the specific cut sites.)
Each answer may be used once, more than once, or not at all.
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