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28 Sep 2019
A student working with E. coli wants to run an experiment on a population that is as dense as possible without actually going into stationary phase. In Luria- Bertani broth, commonly used to grow E. coli, cells begin entering stationary phase at a cell density of
5 Ã 107 cells/mL. She inoculates a 10 mL culture with 1000 cells and sets it up in a
shaking incubator, knowing that after about an hour of lag time E. coli should double every
20 minutes. How long can she go do something else before she has to get back for her
experiment?
A student working with E. coli wants to run an experiment on a population that is as dense as possible without actually going into stationary phase. In Luria- Bertani broth, commonly used to grow E. coli, cells begin entering stationary phase at a cell density of
5 Ã 107 cells/mL. She inoculates a 10 mL culture with 1000 cells and sets it up in a
shaking incubator, knowing that after about an hour of lag time E. coli should double every
20 minutes. How long can she go do something else before she has to get back for her
experiment?
Nelly StrackeLv2
28 Sep 2019
