IMVic (Indole, Methyl Red, Voges-Prokauer, and Cietrate) Reactions
Name___________________________________________________________ Date _________________________
Record your results after incubation in the table.
SIM
MRVP
Citrate
Organism
Sulfide
(+ or -)
Indole
(+ or -)
Motility
(+ or -)
MR
(+ or -)
VP
(+ or -)
Slant
Color
Citrate
(+ or -)
Salmonella
typhimurium
NA
NA
NA
NA
Escherichia
coli
Enterobacter
aerogenes
NA
NA
NA
Klebsiella
pneumonia
NA
NA
NA
NA
Interpretation and Questions:
The IMViC tests are based on some enzymatic/physiological process. Name the substrates for the enzymes in the indole, MRVP reactions, and citrate test.
Is it possible for an organism to be MR+ and VP+? Why or why not?
Why is it important to inoculate only a single tube per organism when performing the MRVP test?
During lab, your lab partner bumps into your, jostling your arm while you are inoculating your SIM tube. Is it still possible to reliably evaluate the sulfide, indole, and motility results from this tube? Which reactions will still be accurate?
The case study describes a cluster of food poisoning incidences. If the causative agent is Salmonella spp., then give your prediction of the IMViC test results.
IMVic (Indole, Methyl Red, Voges-Prokauer, and Cietrate) Reactions
Name___________________________________________________________ Date _________________________
Record your results after incubation in the table.
| SIM | MRVP | Citrate | |||||
| Organism | Sulfide (+ or -) | Indole (+ or -) | Motility (+ or -) | MR (+ or -) | VP (+ or -) | Slant Color | Citrate (+ or -) |
| Salmonella typhimurium | NA | NA | NA | NA | |||
| Escherichia coli | |||||||
| Enterobacter aerogenes | NA | NA | NA | ||||
| Klebsiella pneumonia | NA | NA | NA | NA | |||
Interpretation and Questions:
The IMViC tests are based on some enzymatic/physiological process. Name the substrates for the enzymes in the indole, MRVP reactions, and citrate test.
Is it possible for an organism to be MR+ and VP+? Why or why not?
Why is it important to inoculate only a single tube per organism when performing the MRVP test?
During lab, your lab partner bumps into your, jostling your arm while you are inoculating your SIM tube. Is it still possible to reliably evaluate the sulfide, indole, and motility results from this tube? Which reactions will still be accurate?
The case study describes a cluster of food poisoning incidences. If the causative agent is Salmonella spp., then give your prediction of the IMViC test results.
