Medical testing: enzyme linked immunosorbent assay (ELISA) test for presence of antigen or antibody
EXPERIMENTAL PROTOCOL
In this laboratory, you will test seven different patients using a direct ELISA. We have access to serum samples from the main characters of the TV show The Big Bang Theory. You will test each of them for the presence of ZIKA virus and HIV, as well as perform a pregnancy test, using known antigens/antibodies for those diseases/test. Students will work in pairs. Serum for each character of the Big Bang Theory has been incubated overnight at 4°C in the wells of an ELISA plate.
Testing Control Sheldon Penny Leonard Raj Bernadetts Howard Amy Zika + + + + hCG (pregnancy) + + HIV + +
1) Interpretations: Summarize the interpretation of the findings about the results and the experiment
2) Future Scientific Directions: suggest future experiments using as base the results obtained in the lab
3) Create a question to the lab experiement approach.
5) Approach: Create a summary of the overall lab experiment. Hypothesis.
Medical testing: enzyme linked immunosorbent assay (ELISA) test for presence of antigen or antibody
EXPERIMENTAL PROTOCOL
In this laboratory, you will test seven different patients using a direct ELISA. We have access to serum samples from the main characters of the TV show The Big Bang Theory. You will test each of them for the presence of ZIKA virus and HIV, as well as perform a pregnancy test, using known antigens/antibodies for those diseases/test. Students will work in pairs. Serum for each character of the Big Bang Theory has been incubated overnight at 4°C in the wells of an ELISA plate.
| Testing | Control | Sheldon | Penny | Leonard | Raj | Bernadetts | Howard | Amy |
| Zika | + | + | + | + | ||||
| hCG (pregnancy) | + | + | ||||||
| HIV | + | + |
1) Interpretations: Summarize the interpretation of the findings about the results and the experiment
2) Future Scientific Directions: suggest future experiments using as base the results obtained in the lab
3) Create a question to the lab experiement approach.
5) Approach: Create a summary of the overall lab experiment. Hypothesis.
