🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
Biology
1
answer
0
watching
137
views

Please answer

what are endospores and describe ( structure and function) and Why might endospores give a species of bacteria a competitive advantage and What primary and secondary stain will we be using during the endospore stain?

Watch
1
answer
0
watching
137
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Patrina Schowalter
Patrina SchowalterLv2
28 Sep 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

Delia, a five-year-old, woke up one morning complaining that she had a sore throat, headache and stomachache. Her tonsils appeared swollen, and a tonsular exudate, appearing as a cream-colored pus, was evident upon examining the child’s oropharynx. Her mother also took Delia’s temperature and noted the girl was running a low-grade fever. Suspecting strep throat (streptococcal pharyngitis), her mother made an appointment with the pediatrician.

The doctor first performed a rapid strep test, which works by detecting certain cell surface proteins on group A streptococci (GAS). This test is inexpensive and can detect GAS in a matter of minutes. However, rapid strep tests that come back negative are not especially reliable; about five out of every 100 patients with streptococcal pharyngitis will have a negative rapid strep test result. Therefore, despite Delia’s rapid strep test being negative, the doctor went ahead and ordered a bacterial culture. This was a wise decision because Delia had signs and symptoms that pointed to a case of streptococcal pharyngitis caused by the Gram-positive, nonmotile, encapsulated prokaryote Streptococcus pyogenes.

When cultures to detect S. pyogenes are performed, the patient’s sample (in this case a swab of the throat) is streaked out onto a specialized nutrient-rich agar called blood agar. Actively growing S. pyogenes can break down red blood cells (a process called beta hemolysis), and, therefore, is readily detectable on blood agar plates because a clear zone develops around its colonies. Based on the microbiology data that came back, Delia was treated for streptococcal pharyngitis. After 48 hours on an antibiotic, Delia felt much better and returned to school.

QUESTION: Would you expect to find endospores if you conducted an endospore stain of the patient’s cultured infectious agent? Explain your reasoning. (Choose the single best answer.)

A. Endospores should be found within the patient’s culture since S. pyogenes makes endospores in response to antibacterial drugs treatments.
B. Endospores should be found within the patient’s culture since S. pyogenes was grown in nutrient-limiting conditions.
C. Endospores should not be detected in the patient’s culture since S. pyogenes is a non-endospore forming pathogen.
D. Endospores should not be detected in the patient’s culture since S. pyogenes was grown under favorable conditions that promote germination.
E. Endospores should not be detected in the patient’s culture since S. pyogenes is Gram-positive, and, therefore, doesn’t make endospores.

1) Immunostaining. Using anti-zip8 for cell staining, what doesit stain?

2) how do we visualize and analyize results while performingcell staining with DAPI and anti-zip8? What do the results ofstaining imply?

3) Immunostaining (what is primary antibody? What is secondaryantibody? Why we need a secondary antibody?)

4) H&E stain of mouse liver. what is the morphology ofnormal liver? what is the morphology of abnormal liver? what is thepathology?

5) Immunostaining (what is primary antibody? What is secondaryantibody? Why we need a secondary antibody?)

1. When subjected to the Gram stain, mycobacteria either stain weakly red or don’t stain at all. Explain why, describing the differences between the cell envelopes of acid-fast, gram positive, and gram negative bacteria.

2. Synthesizing cell walls with mycolic acids requires a great deal of energy. Do you expect acid-fast bacteria to grow fast or slow compared to other bacteria? Explain.

3. What are the chemical differences between the Gram stain and the acid fast stain?

4. Describe how the LIVE/DEAD stain distinguishes between living and dead cells.

5. What results would you expect from spores? Explain your answer.

6. How can this stain be used to get an accurate measure of living vs. dead cells?

7. Develop a hypothesis that can be tested using the LIVE/DEAD stain

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in