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For Microbiology, I am doing a lab and am stuck on two of thequestions. It is 1-4 Common Aseptic Transfers and InoculationMethods.

1) Which medium was most difficult for you to transfer from?Which medium was most difficult for you to inoculate? Explain yourdifficulties. (Note: there are no current answers to thesequestions, but based on your evaluation of personalexperiences)

2) Did you get growth on/in the sterile NB and NA slant tubesyou practiced with? If so, where did you see it and what might havebeen its source(s)?

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Sixta Kovacek
Sixta KovacekLv2
28 Sep 2019

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Related questions

1. Should you see signs of microbial growth in a sterile medium if you allow it to incubate for several days? Explain your answer.

2. Do you think there were any deficiencies in your aseptic technique, and if so, can you identify them? Explain your answer. If there were no deficiencies in your aseptic technique, identify at least one potential way through which contamination could occur while working with cultures.

3. Which was most challenging for you in terms of aseptic technique: transfer from broth to broth, broth to slant, or broth to plate? Explain your answer.

2. Which of the following would the MSA medium NOT be considered: Select one:

a. Complex medium

b. Chemically defined medium

c. Differential medium

d. Selective medium

3. If you took a swab from a patient and cultured it on a MSA plate, which of the following could be identified by just using this test? Select one:

a. Staphylococcus epidermidis

b. Escherichia coli

c. Staphylococcus aureus

d. Micrococcus luteus

4. If you streaked the 3 organisms in this lab on a PEA plate and after incubating for 2 days at 37 degrees Celcius nothing grew (but they did grow on a NA plate made at the same time and grown in the same conditions), which of the following is the most likely cause for this result? Select one:

a. The agar was toxic.

b. The beef extract was bad.

c. The temperature was too low.

d. Too much phenylethyl alcohol was used

. e. The temperature was too high.

5. Why is the NA plate inoculated in addition to the Columbia CNA with 5% sheep blood agar plate? Select one:

a. Used to compare growth on non-selective medium

b. Used as second selective medium

c. Used to promote hemolysis

d. Used because it is easier to see

e. Used as a practice plate

Considering the cultures to be used for inoculating each medium in this exercise, how many microbial types should you expect to see in/on each medium?

Select one:

a. 3

b. 0

c. 1

d. Several

e. 2

Which of the following is common to minimize airborne contamination?

Select one:

a. Make someone else do the experiment

b. Perform the experiment in a windy room

c. Keep a microscope nearby

d. Breathing in sample

e. Hold open tubes at an angle

How can a hot sterile flame loop be cooled quickly to use in the next streak?

Select one:

a. Put in sterile portion of agar plate

b. Blow on it

c. Stick the loop in ice

d. Set the loop on the lab bench

e. Pour tap water on the loop

True or false: Most colonies can been seen without aid (naked eye), and most single cells cannot be seen without a microscope.

Select one:

True

False

An isolated, single colony forms after incubation of a:

Select one:

a. Virus (viral particle arrangement in some cases)

b. Single cell (cell arrangement in some cases)

c. Sterile agar plate

d. Sterile flame loop

e. Liquid mixed culture

These are commonly used for growing stock cultures that can be refrigerated for several weeks:

Select one:

a. Broth tubes

b. Cotton swabs

c. Gym socks

d. Agar slants

e. Petri dishes

Beginning at the base of the slant surface and gently moving the loop back and forth as you withdraw is called:

Select one:

a. Slant mixing

b. Fishtail inoculation

c. Zig-zag streaking

d. Squirrel inoculation

e. Quadrant streaking

If you are going to mix a broth culture prior to transfer, what is a safe way to do this without special equipment?

Select one:

a. Tap the tube with your fingers

b. Vortex it

c. Use a inoculating loop

d. Stick the tube in the outer cone

What is the primary purpose of the streak plate method?

Select one:

a. Quantify CFUs

b. Isolate an individual colony

c. Quantify the cell count of a mixed culture

d. Inoculate a culture on a plate

e. Confirm the identity of an unknown genus

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