A polysome isa.the assembly at a promoter at the start of transcription. b.an mRNA with multiple ribosomes attached. c.the complex that puts a poly(A) tail on an mRNA. d.the combination of a large and a small ribosome subunit. e.the complex where mRNA splicing occurs.
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QUESTION 1
In the bacterial lac repressor, which of these occurs: (select all that apply)
| (a)In the presence of glucose and the absence of lactose, the repressor is actively produced. | ||
| (b)The lac operon can be hyper-induced in the presence of low glucose by the 'cAMP binding protein' (CAMP). | ||
| (c)In the presence of lactose and the absence of glucose the lac operon is repressed. | ||
| (d)Allolactose is a byproduct of β-galactosidase, indicating high lactose when it builds up. |
1.5 points
QUESTION 2
In eukaryotes the enhancer elements can be quite a distance upstream or downstream from the promoter, and transcriptional regulation is helped by protein:protein interactions.
True
False
1.5 points
QUESTION 3
The prokaryotic promoter contains a TATA box that is at -10bp upstream from the transcriptional start site.
True
False
1.5 points
QUESTION 4
During the addition of the poly A tail which of these occurs:(select all that apply)
| (a)The transcript is synthesized beyond the poly A site (AAUAAA). | ||
| (b)The mRNA contains a 5â cap. | ||
| (c)The transcript is cleaved 35nt downstream from the poly A site. | ||
| (d)An enzyme complex containing an exonuclease and polyadenylate polymerase cleaves nearby the AAUAA site. | ||
| (e)100-300 Aâs are added to the 5â end. |
