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PLEASEREAD THE QUESTION CAREFULLY AND ANSWER ALL QUESTIONS. THANKYOU!

Youhave cloned and sequenced a gene using the dideoxy sanger method.How do youdetermine DNA nucleotides from a template DNA sequence startingfrom the 3' end of the template on a dideoxy sangerautoradiogram.

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Collen Von
Collen VonLv2
28 Sep 2019

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Related questions

1. You are sequencing a 350bp lizard gene using the Sanger dideoxy sequencing method. You have amplified the region using the primer 5'-ATCCGGGCG-3'. What will be the length of the lowest band in your sequencing gel?

2. Which of the following answer(s) are true (select all that apply)?

Question options:

PCR results in exclusively the desired region to be amplified.

PCR primers must be outside the region of interest. PCR primers must be flanking the region of interest.

Sequencing primers must be flanking the region of interest.

All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of interest.

Sanger sequencing requires dNTPs.

A standard PCR reaction requires ddNTPs Small DNA fragments on a gel would be closer to the wells on a gel than larger DNA fragments.

1) Which of the following is FALSE about plasmid vectors ued in recombinant DNA research?
A) Usually carrry an antibiotic resistance gene
B) Clonin sites are always located between genes, that is, outside coding sequences
C) May hae a multiple clonin gsite which can be cut by several restriction enzymes
D) May be expression vectors

2) Which of the following is FLASE about Sanger Dideoxy DNA sequencing protocols?
A) Different specfiic chemical reactions break the DNA at each base.
B) Fluorescent molecules can be used to detect the DNA
C) The Template is single stranded DNA
D) Many steps can be automated

3) A southern blot transfers ___ to a Nitrocellulose membrane.
A) Protein
B) RNA
C) DNA
D) Lipid

4) Which of the following is true about cDNA?
A) It is made from DNA template
B) It is isolated from bacteria
C) Reverse transcriptase is used to synthesize cDNA
D) It has introns

5) The restriction endonucleases used in recombinant DNA work:
A) Always produce blunt ends (No unpaired bases)
B) Recognize sequences 4-6 bp long
C) Cut the DNA outisde the recognition sequence
D) All of the above

S17

THANK YOU VERY MUCH!!!

Question 1. You are sequencing a 350bp lizard gene using the Sanger dideoxy sequencing method. You have amplified the region using the primer 5'-ATCCGGGCG-3'. What will be the length of the lowest band in your sequencing gel?

(A) Cannot answer the question with the provided information

(B) 350

(C) 10

(D) 9

(E) 351

Question 2. True or False

If cell has a mutated gyrase, it means that it will be unable to properly join together okazaki fragments and therefore will result in many fragmented pieces of DNA.

Question 3. Which of the following answer(s) are true (select all that apply)?

- PCR results in exclusively the desired region to be amplified.

- PCR primers must be outside the region of interest.

- PCR primers must be flanking the region of interest.

- Sequencing primers must be flanking the region of interest.

- All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of interest.

- Sanger sequencing requires dNTPs.

- A standard PCR reaction requires ddNTPs

- Small DNA fragments on a gel would be closer to the wells on a gel than larger DNA fragments.

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