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BACTERIAL GROWTH AND CONTROLLING BACTERIALGROWTH

1. Based upon your results, is your bacteria sample a strictaerobe, strict anaerobe, facultative anaerobe, or microaerophile?Why? – Look at your results from OF Glucose and OF Glucose with oiltest.

2. Did the bacteria grow in the tryptone or malonate media? Whyor why not? – Remember, you can tell if they grew if culture wasturbid (cloudy).

3. For the media used in this portion of the experiment, are themedia complex or synthetic? Are the media selective, differential,both, or neither? Identify the carbon and nitrogen source for eachmedium. – Read more about what these terms mean starting on page178 in your book right below Figure 6.11. Look up each media withthe M? button. Remember, nutrient broth is a complex media, mineralsalts is a defined media.

4. How many possible bacteria were remaining after conductingthese biochemical tests?

5. Using the information in the Identification matrix, whatadditional tests might help you to identify the bacteria in yourcase study? –Look at the View Identification Matrix under the Viewmenu. What tests would you perform? Look for ones that have a mixof + and – because they would help distinguish betweenorganisms.

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Lelia Lubowitz
Lelia LubowitzLv2
28 Sep 2019

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