Question

1. As you continue on in your career, you become keenly interested inthe mechanisms of immune cell recruitment during the innate immuneresponse. After years of graduate work, you discovered a newcytokine (X), which chemoattracts neutrophils. In your excitementyou begin devising experiments to characterize the function andrelevance of cytokine X. (30 pts)
   1. From what you know of chemokines, predict the general structure ofchemokine X and its cognate receptor.
   2. Where, by what cell types, and when would you expect it to beexpressed? Explain, experimentally, how you would test yourhypothesis.
   3. What is the effect of expression of chemokine X on the immuneresponse. Explain, experimentally, how you would test yourhypothesis.

1. As you are working in the lab, you obtain a major career-definingresult. You have discovered a new TLR (TLR15), which recognizeseither a bacterial or viral antigen. Your task to finish graduateschool is to fully characterize the molecule. (30 pts)
   1. Design an experiment to determine if your new TLR15 recognizes abacteria or a virus. What results do you anticipate and whatexperimental difficulties will you potentiallyencounter?
   2. Comment on the likely structure for the molecule and indicate aplausible signaling pathway for cellular activation.
   3. Where in the cell would you expect to find this new TLR? Why?Please design and fully explain two different experiments to testyour hypothesis.
   4. What are the consequences of deficiency of TLR15? Please design andfully explain two different experiments to test yourhypothesis.