Amino acid analysis of a decapeptide revealed the presenceof:
Asp Glu Tyr Arg Met Pro Lys Ser Phe
The following facts were observed:
Carboxypeptidase A or B treatment of the decapeptide had anyeffect
Trypsin treatment yielded two tetrapeptides and free Lys
Clostripain treatment yielded a tetrapeptide and ahexapeptide.
Cyanogen bromide treatment yielded an octapeptide and adipeptide of sequence Asn and Pro.
Chymotripsin treatment yielded two tripeptides and atetrapeptide. The N terminal chymotryptic peptide had a charge of-1 at neutral pH and a net charge of -3 at pH=12.
One cycle of Edman degradation gave PTH-Ser.
What is the amino acid sequence?
Amino acid analysis of a decapeptide revealed the presenceof:
Asp Glu Tyr Arg Met Pro Lys Ser Phe
The following facts were observed:
Carboxypeptidase A or B treatment of the decapeptide had anyeffect
Trypsin treatment yielded two tetrapeptides and free Lys
Clostripain treatment yielded a tetrapeptide and ahexapeptide.
Cyanogen bromide treatment yielded an octapeptide and adipeptide of sequence Asn and Pro.
Chymotripsin treatment yielded two tripeptides and atetrapeptide. The N terminal chymotryptic peptide had a charge of-1 at neutral pH and a net charge of -3 at pH=12.
One cycle of Edman degradation gave PTH-Ser.
What is the amino acid sequence?
Amino acid analysis of a decapeptide revealed the presenceof:
Asp Glu Tyr Arg Met Pro Lys Ser Phe
The following facts were observed:
Carboxypeptidase A or B treatment of the decapeptide had anyeffect
Trypsin treatment yielded two tetrapeptides and free Lys
Clostripain treatment yielded a tetrapeptide and ahexapeptide.
Cyanogen bromide treatment yielded an octapeptide and adipeptide of sequence Asn and Pro.
Chymotripsin treatment yielded two tripeptides and atetrapeptide. The N terminal chymotryptic peptide had a charge of-1 at neutral pH and a net charge of -3 at pH=12.
One cycle of Edman degradation gave PTH-Ser.
What is the amino acid sequence?
Amino acid analysis of a decapeptide revealed the presenceof:
Asp Glu Tyr Arg Met Pro Lys Ser Phe
The following facts were observed:
Carboxypeptidase A or B treatment of the decapeptide had anyeffect
Trypsin treatment yielded two tetrapeptides and free Lys
Clostripain treatment yielded a tetrapeptide and ahexapeptide.
Cyanogen bromide treatment yielded an octapeptide and adipeptide of sequence Asn and Pro.
Chymotripsin treatment yielded two tripeptides and atetrapeptide. The N terminal chymotryptic peptide had a charge of-1 at neutral pH and a net charge of -3 at pH=12.
One cycle of Edman degradation gave PTH-Ser.
What is the amino acid sequence?
