Conditional mutations are useful for genetic analysis ofessential processes. For example, temperature- sensitive cell-cyclemutations in yeast do not divide at 37

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Trinidad TremblayLv2

28 Sep 2019

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Conditional lethal mutants have proven indispensible in genetic and biochemical analyses of complex processes such as DNA replication. Temperature-sensitive (ts) mutations, which are one form of conditional lethal mutation, allow growth at one temperature (for example, 30oC) but not at a higher temperature (for example, 42oC). A large number of ts mutants have been isolated in E. coli. These mutant bacteria are defective in DNA replication at 42oC but not at 30oC. If the temperature of the medium is raised from 30oC to 42oC, these mutants stop making DNA in one of two characteristic ways. The 'quick stop' mutants halt DNA synthesis immediately, whereas the 'slow stop' mutants stop DNA synthesis only after a few minutes. Which of the following mutant proteins would you predict would display a 'quick stop' phenotype?

a. DNA topoisomerase I, a replication initiator protein, DNA ligase, and DNA helicase

b. DNA topoisomerase I, single-stranded DNA-binding protein, DNA ligase, and DNA helicase

c. DNA topoisomerase I, single-stranded DNA-binding protein, DNA primase, and DNA helicase

d. A replication initiator protein, single-stranded DNA-binding protein, DNA ligase, and DNA helicase

e. DNA topoisomerase I, single-stranded DNA-binding protein, DNA ligase, and DNA helicase

In another yeast strain, conditionally lethal mutations havebeen identified in the mad gene. At temperatures above43^oC, the cell dies. Nuclear analysis of the mutants,indicates that at higher temperatures the sister chromatids fail toseparate during anaphase of mitosis. Propose a model for whathappening in the cell as the temperature is raised to43^oC

champagnellama936

You have isolated a strain of temperature-sensitive mutant yeast that divides normally at 25Â°C. However, at 37Â°C, the mutant pauses in the cell cycle right before M phase. These mutant yeast cells are NOT defective in MCdk. For each one of the following conditions, state whether or not it could be responsible for the behavior of this strain of yeast (3 points). Explain your reasoning for each choice.

A. Inactivation of an enzyme that degrades M-Cyclin.

B. Inactivation of the Wee-1 kinase.

C. Inactivation of the Cdc25 phosphatase