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1. How does the cell initiate DNA replication at an origin, yet prevent a second round of replication at that same origin?

2. How do higher eukaryotes recognize an origin?

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Reid Wolff
Reid WolffLv2
28 Sep 2019

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Related questions

1.When does DNA replication occur in the life of a prokaryotic cell? When does it take place in the life of a eukaryotic cell? Is it always for the same purpose?

2.What proteins are necessary for DNA replication and what are their functions?

3.What is an origin of replication? How does it differ in prokaryotes and eukaryotes?

4.Why are primers needed and how are they made? What is lagging strand synthesis and why is it necessary?

5.What is the difference between a replication fork and a replication bubble?

5. A) DnaB is a helicase that loads as a ring onto one strand ofthe DNA and pushes it apart from the second strand. This allows for5’-3’ DNA unwinding and replication fork advancement that isnecessary for initiation and elongation. If you create atemperature sensitive mutation in DnaB, what will be the effect onDNA replication (quick stop/slow stop mutation) at thenon-permissive temperature? Why does this occur?

B) DnaA is a protein that binds to OriC (origin of replication)to initiate DNA replication. This protein must be made new witheach round of replication. If you create a temperature sensitivemutation in DnaA, what will be the effect on DNA replication (quickstop/slow stop) at the non-permissive temperature? Why does thiseffect occur?

Describe the various steps involved in the initiation of replication in eukaryotes. What proteins are involved in each of the steps, and when in the cell cycle does each of them occur? How does their temporal segregation contribute to the limitation of origin firing to at most once per cell cycle?

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