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28 Sep 2019
1.) What is the cellular role of glutathione and how is it relatedto NADPH?
----Many metabolic expirements are performed using substratescontianing radioactive isotopes such as 14^C. Substrates, forexample gluccose, can be synthesized with radioactive isotopes inknown locations within a molecule Specifically, 6 differentgluccose molecules could be made with different carbon labeled ineach position. It is known that gluccose can be metabolized to avariety of products in cluding pyruvate,carbon dioxide, and othersthrough several different pathways such as glycolysis and thecitric acid cycle. By knowing which carbon is labeled in a givenexpirement, scientist can learn something about metabolic pathwaysused under given conditions by detecting radiolabeled substratesfor a finite period of time after which the desired metabolicproduct, for example carbon dioxide, would be collected andmeasured for radioactivity.
2.) Which carbon would need to be labeled in gluccos in order forit to be detected in carbon dioxide as an indicator of gluccoseentry into the pentose phosphate pathway?
1.) What is the cellular role of glutathione and how is it relatedto NADPH?
----Many metabolic expirements are performed using substratescontianing radioactive isotopes such as 14^C. Substrates, forexample gluccose, can be synthesized with radioactive isotopes inknown locations within a molecule Specifically, 6 differentgluccose molecules could be made with different carbon labeled ineach position. It is known that gluccose can be metabolized to avariety of products in cluding pyruvate,carbon dioxide, and othersthrough several different pathways such as glycolysis and thecitric acid cycle. By knowing which carbon is labeled in a givenexpirement, scientist can learn something about metabolic pathwaysused under given conditions by detecting radiolabeled substratesfor a finite period of time after which the desired metabolicproduct, for example carbon dioxide, would be collected andmeasured for radioactivity.
2.) Which carbon would need to be labeled in gluccos in order forit to be detected in carbon dioxide as an indicator of gluccoseentry into the pentose phosphate pathway?
----Many metabolic expirements are performed using substratescontianing radioactive isotopes such as 14^C. Substrates, forexample gluccose, can be synthesized with radioactive isotopes inknown locations within a molecule Specifically, 6 differentgluccose molecules could be made with different carbon labeled ineach position. It is known that gluccose can be metabolized to avariety of products in cluding pyruvate,carbon dioxide, and othersthrough several different pathways such as glycolysis and thecitric acid cycle. By knowing which carbon is labeled in a givenexpirement, scientist can learn something about metabolic pathwaysused under given conditions by detecting radiolabeled substratesfor a finite period of time after which the desired metabolicproduct, for example carbon dioxide, would be collected andmeasured for radioactivity.
2.) Which carbon would need to be labeled in gluccos in order forit to be detected in carbon dioxide as an indicator of gluccoseentry into the pentose phosphate pathway?
Jean KeelingLv2
28 Sep 2019
