Q1. A researcher tests two different restriction enzymeson pUC18, performing the pUC18 digests then running them in anagarose gel against linear standards. The first enzyme produced theexpected single linear band of ~2.7 kb, but the second digestproduced a single band of only about 0.85 kb. (a) Explain thissecond digest result. (b) What telltale clue in the gel profilewould lead you to the correct answer for part (a)?
Q2. A DNA fragment, obtained from a partial SmaI digestof a longer fragment, is ligated into the unique SmaI site of thecloning vector, pUC18. A subsequent and complete SmaI digest of newrecombinant plasmid yielded a total of four SmaI fragments. Howmany SmaI sites are there in this recombinant plasmid? How manySmaI sites were in the original partially digested fragment(careful!)?
Clue for Q2: Therecombinant plasmid has
Q1. A researcher tests two different restriction enzymeson pUC18, performing the pUC18 digests then running them in anagarose gel against linear standards. The first enzyme produced theexpected single linear band of ~2.7 kb, but the second digestproduced a single band of only about 0.85 kb. (a) Explain thissecond digest result. (b) What telltale clue in the gel profilewould lead you to the correct answer for part (a)?
Q2. A DNA fragment, obtained from a partial SmaI digestof a longer fragment, is ligated into the unique SmaI site of thecloning vector, pUC18. A subsequent and complete SmaI digest of newrecombinant plasmid yielded a total of four SmaI fragments. Howmany SmaI sites are there in this recombinant plasmid? How manySmaI sites were in the original partially digested fragment(careful!)?
Clue for Q2: Therecombinant plasmid has
