For each of the scenarios below use this list of possibilities to choose the most likely unknown organism.
Consider the following possibilities: E. coli, K rhizophila, S. marcescenes, S. aureus, B. subtilis,
S. epidermidis, S. pyogenes, M. luteus, E. aerogenes, P. aeruginosa, P. vulgaris.
Scenario-1
Even though we did not use Mannitol Salt media in our experiment, knowing what you do about its properties predict the unknown for this scenario. Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed, on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared white.
Organism = _________________________________________
Scenario-2
Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared yellow.
Organism = __________________________________________
Scenario-3
Data: microscopic observation at 1000X = small individual rods, growth on nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of metallic green colonies.
Organism = __________________________________________
Scenario-4
Data: microscopic observation at 1000X = individual rods, growth on Nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of thick, mucoid, pink colonies.
Organism = ___________________________________________
Scenario-5
Data: microscopic observation at 1000X = rods in chains, diplo bacilli and individual rods, growth on Nutrient Agar, and PEA media, no growth was observed on Mannitol Salt or EMB media.
Organism = ____________________________________________
1)Could you identify your unknown from the Gram Stain alone? _____ How did you identify it?
For each of the scenarios below use this list of possibilities to choose the most likely unknown organism.
Consider the following possibilities: E. coli, K rhizophila, S. marcescenes, S. aureus, B. subtilis,
S. epidermidis, S. pyogenes, M. luteus, E. aerogenes, P. aeruginosa, P. vulgaris.
Scenario-1
Even though we did not use Mannitol Salt media in our experiment, knowing what you do about its properties predict the unknown for this scenario. Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed, on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared white.
Organism = _________________________________________
Scenario-2
Data: microscopic observation at 1000X = cocci in cluster arrangement, Growth was observed on Nutrient agar, PEA media and Mannitol Salt media while no growth was seen on EMB media. Colonies on Mannitol Salt appeared yellow.
Organism = __________________________________________
Scenario-3
Data: microscopic observation at 1000X = small individual rods, growth on nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of metallic green colonies.
Organism = __________________________________________
Scenario-4
Data: microscopic observation at 1000X = individual rods, growth on Nutrient Agar, EMB media, but no growth on PEA media. Growth appearance on EMB was that of thick, mucoid, pink colonies.
Organism = ___________________________________________
Scenario-5
Data: microscopic observation at 1000X = rods in chains, diplo bacilli and individual rods, growth on Nutrient Agar, and PEA media, no growth was observed on Mannitol Salt or EMB media.
Organism = ____________________________________________
1)Could you identify your unknown from the Gram Stain alone? _____ How did you identify it?
Related textbook solutions
Related questions
microbiology double unknown
1. G+ coccus
*tiny colonies
*white colony
*sediment
*Phenol Red:
PR Glu: (A/-) Yellow
PR Lac: (A/-) Yellow
PR Suc: (A/-) Yellow
*SIM test
Sulfure reduction: (-) No black
Indol: (-) No pink
Motility: (+)
*MR: (+) Red
*VP: (+) Red (redish brown)
* Red blood agar: beta hemo
*MacConkey: (-) No growth (G+)
*DNAse: (+) clearing around
*Gelatin: (+) liquid in 5 days
*Nitrate reduction: (+) red when added 2 indicators
*Citrate: (-) green
*Casein: (-) unclear
* EMB: (-) no growth
*MSA: (+) with yellow halo
*starch: (-) no clear around
2. Gram - bacillus
*tiny colonies
*yellow and sometimes red color inside of colony
*colony forming unit: Flocculent
*oxidase: (-) no blue
*Phenol Red
PR Glu: (A/-) Yellow
PR Lac: (-/-) No color change
PR Suc: (A/-) Yellow
*SIM test
Sulfur reducation: (-) No black
Indole: (-) no pink
motility: [+] little growth inside
*MR: (-) No color change
*VP: (+) red (red on the top and yellow on the bottom)
*Red blood cell Agar: alpha hemo
*MacConkey: G- and no color change (No fermentt lactose/noncoliform)
*DNAse: (+) clearing around
*Gelatin: (+) in 1 day
*Nitrate: (+) red after adding indicators
*Citrate: (+) blue
*Casein: (+) clear ........... (NOT SURE)
*EMB: poor growth
*MSA: (+) with yellow halo
*TSI: (A/A)(NG) yellow top and bottom, no gas
*Starch: (+) clear around after adding indicator
What is thename of Gram+ and Gram- Bacteria?
My instiructor gave us this bacteria list (he said someunknown bacteria can be not on the list)
| B. Cereus |
| E. coli |
| E. aerogenes |
| E. faecalis |
| Streptococcus bovis |
| K. pneumoniae (pneumoniae |
| S. aureus |
| S. epidermidis |
| Moraxella Moratella Osloensis |
| P. vulgaris |
| B. subtilis |
| M. luteus |
| P. aeruginosa |
| Bacillus Alvei |
| S. marcescens |
| A. faecalis |
| Staphylococcus Saprophyticus |
| Alcaligenes latus |
| Morganella morganii |
| S. sonnei |
| C. xerosis |
| Listeria innocua |
| Proteus mirabilis |
| Streptococcus agaloctiae |
| Salmonella Choleraesais |
| S. mitis |
