Log in
Biology
2
answers
0
watching
88
views

A student drags a cotton swab through the zone of inhibition from a Kirby Bauer test and uses it to inoculate a new streak plate. They observed growth on their streak plate, meaning that the antibiotic being tested was: (bacteriostatic or bactericidal?) __________________This means that the antibiotic:___________________

Watch
2
answers
0
watching
88
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share
Tod Thiel
Tod ThielLv2
28 Sep 2019
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

I need help with the discussion and conclusion. This is the correct result from the experiment and correct format for the lab report.

Objective

Antibiotic disc test (Perform antibiotics test assay)

Introduction

The purpose of this experiment is to learn how to test the susceptibility of microorganisms to various antibiotics. Antibiotic is a chemical produced by a fungus or bacterium that has the potential to limit the growth of another bacterium.

Material and Methods

The description is in the laboratory manual. Test 1 using the aseptic technique to paint the entire surface of a sterile agar plate with E. coli and to covers all the areas then place four different antibiotics disc onto the marked surface with their proper name onto it.

Results

Table 1: Antibiotic inhibition zone results for E. coli

Diameter – Disc/2 = Radius (r)

Disc = 6mm/10 = 0.6 cm.

Bacterium: E. coli

Antibiotic used

Inhibition zone size (cm)

Penicillin 10 mg/1

0

Ampicillin

3.8 – 0.6/2 = 1.6

Penicillin 100 mg/1

1.70 – 0.6/2 = 0.55

Cefoxitin

4.20 – 0.6/2 = 1.8

Discussion/conclusion

1. The two genera, Bacillus and Clostridium, are similar in that both are

a. catalase positive.

b. strict anaerobes.

c. spore formers.

d. strict pathogens.

ANSWER:

(Level 1, Objective 1)

2. A physician suspects her patient has erysipeloid. She calls the laboratory for help with specimen selection and collection. You recommend she collect a

a. swab of infected skin surface.

b. nasopharyngeal swab.

c. swab of sinus tract drainage.

d. full thickness biopsy.

ANSWER:

(Level 1, Objective 2)

3. To diagnose diphtheria, the appropriate specimen for collection is a

a. pharyngeal swab.

b. portion of the membrane.

c. portion of the black scab.

d. swab of the contaminated medical device.

ANSWER:

(Level 1, Objective 2)

4. Loeffler’s medium is useful in making a presumptive identification of Corynebacterium diphtheriae from a pharyngeal culture because it

a. inhibits the growth of normal upper respiratory flora.

b. detects hydrogen sulfide production.

c. enhances metachromatic granule formation.

d. detects toxin-producing strains.

ANSWER:

(Level 1, Objective 3)

5. Overlaying an agar plate with sterile olive oil ensures adequate growth of

a. Erysipelothrix rhusiopathiae.

b. Arcanobacterium haemolyticum.

c. Lactobacillus spp.

d. Corynebacterium urealyticum.

ANSWER:

(Level 1, Objective 3)

6. The breakdown of casein, tyrosine, and xanthine is used in differentiating ____________ spp.

a. Nocardia

b. Streptomyces

c. Actinomyces

d. Corynebacterium

ANSWER:

(Level 1, Objective 4)

7. A positive reaction for the inverse CAMP test appears as an arrowhead of

a. non-hemolysis for Arcanobacterium haemolyticum when tested against Group B Streptococcus.

b. non-hemolysis for Corynebacterium ulcerans when tested against Staphylococcus aureus.

c. enhanced hemolysis for Arcanobacterium haemolyticum when tested against Group B Streptococcus.

d. enhanced hemolysis for Corynebacterium ulcerans when tested against Staphylococcus aureus.

ANSWER:

(Level 2, Objective 4)

8. Nocardia, Actinomyces, and Streptomyces spp. microscopically appear as

a. palisading, clubbed, gram-positive rods.

b. gram-variable rods.

c. branching gram-positive rods.

d. long, thin, gram-positive rods.

ANSWER:

(Level 1, Objective 5)

9. Lactobacillus spp. typically produce colonies that are

a. white and chalky.

b. large, dry, and beta-hemolytic.

c. gray-white, translucent. with subtle beta-hemolysis.

d. pinpoint and alpha-hemolytic.

ANSWER:

(Level 1, Objective 5)

11. The only catalase-negative, gram-positive rod capable of producing hydrogen sulfide is also

a. intrinsically vancomycin resistant.

b. esculin positive.

c. motile.

d. gelatinase positive.

ANSWER:

(Level 2, Objectives 5 and 6)

12. A pinpoint, gray-white colony is isolated as >100,000 colonies/mL from the urine of an 89-year-old leukemic patient. The colony stains as palisading gram-positive rods. The organism should be

a. catalase negative and urea positive.

b. catalase positive and urea positive.

c. catalase negative and urea negative.

d. catalase positive and urea negative.

ANSWER:

(Level 2, Objective 6)

13. The gram-positive rod that produces an umbrella motility pattern in semisolid medium is also

a. catalase positive.

b. esculin negative.

c. non-hemolytic.

d. sodium hippurate negative.

ANSWER:

(Level 2, Objective 6)

14. The colonies of Streptococcus agalactiae and Listeria monocytogenes are nearly identical. The two organisms can be differentiated by the

a. sodium hippurate hydrolysis test.

b. CAMP factor test.

c. catalase test.

d. diseases they cause.

ANSWER:

(Level 1, Objective 7)

15. A 20-year-old female presents with pharyngitis. The rapid group A Streptococcus screen is negative, and a blood agar plate is inoculated. After 24 hours incubation, a tiny gray colony with a narrow zone of beta-hemolysis is observed. The microbiologist performs a streptococcal serotype for group A and the result is negative. She should

a. perform a Gram stain and catalase test.

b. finalize the culture as “No Group A Streptococcus isolated.”

c. repeat the streptococcal serotype after boiling the organism suspension.

d. perform an ELEK test.

ANSWER:

(Level 2, Objective 7)

17. Which of the following organisms is not considered pathogenic until it has been infected by a bacteriophage?

a. Bacillus anthracis

b. Corynebacterium pseudotuberculosis

c. C. diphtheriae

d. Actinomyces israelii

ANSWER:

(Level 1, Objective 9)

19. The modified ELEK test is used to

a. detect the metachromatic granules of Corynebacterium diphtheriae.

b. identify C. diphtheriae.

c. detect toxin-producing strains of C. diphtheriae.

d. differentiate C. jeikeium and C. urealyticum.

ANSWER:

(Level 1, Objective 9)

20. A leg wound from a 15-year-old male football player yields:

Heavy growth of Staphylococcus aureus

Light growth of Staphylococcus not S. aureus

Light growth of Diphtheroids

Light growth of viridians Streptococcus

The microbiologist should

a. report the Staphylococcus aureus and call the other organisms normal skin flora.

b. report each of the organisms isolated individually.

c. perform further testing on the diphtheroids.

d. report all four organisms as normal skin flora.

ANSWER:

(Level 2, Objective 10)

21. Individuals infected with toxigenic Corynebacterium diphtheriae must be treated with

a. penicillin.

b. DPT vaccine.

c. antitoxin.

d. a and c.

e. b and c.

ANSWER:

(Level 1, Objective 11)

22 A gray-white colony displaying subtle beta-hemolysis is isolated from a cerebrospinal fluid. The organism is a gram-positive rod that is catalase positive, motile at room temperature, and esculin positive. Which patient history is most likely associated with this culture?

a. 45-year-old female weaver

b. 2-day-old infant male delivered at 32 weeks gestation

c. 33-year-old female with recent dental surgery

d. 58-year-old cattle farmer

ANSWER:

(Level 2, Objective 12)

2. A physician suspects her patient has erysipeloid. She calls the laboratory for help with specimen selection and collection. You recommend she collect a

a. swab of infected skin surface.

b. nasopharyngeal swab.

c. swab of sinus tract drainage.

d. full thickness biopsy.

ANSWER:

(Level 1, Objective 2)

4. Loeffler’s medium is useful in making a presumptive identification of Corynebacterium diphtheriae from a pharyngeal culture because it

a. inhibits the growth of normal upper respiratory flora.

b. detects hydrogen sulfide production.

c. enhances metachromatic granule formation.

d. detects toxin-producing strains.

ANSWER:

(Level 1, Objective 3)

5. Overlaying an agar plate with sterile olive oil ensures adequate growth of

a. Erysipelothrix rhusiopathiae.

b. Arcanobacterium haemolyticum.

c. Lactobacillus spp.

d. Corynebacterium urealyticum.

ANSWER:

(Level 1, Objective 3)

6. The breakdown of casein, tyrosine, and xanthine is used in differentiating ____________ spp.

a. Nocardia

b. Streptomyces

c. Actinomyces

d. Corynebacterium

ANSWER:

(Level 1, Objective 4)

7. A positive reaction for the inverse CAMP test appears as an arrowhead of

a. non-hemolysis for Arcanobacterium haemolyticum when tested against Group B Streptococcus.

b. non-hemolysis for Corynebacterium ulcerans when tested against Staphylococcus aureus.

c. enhanced hemolysis for Arcanobacterium haemolyticum when tested against Group B Streptococcus.

d. enhanced hemolysis for Corynebacterium ulcerans when tested against Staphylococcus aureus.

ANSWER:

(Level 2, Objective 4)

8. Nocardia, Actinomyces, and Streptomyces spp. microscopically appear as

a. palisading, clubbed, gram-positive rods.

b. gram-variable rods.

c. branching gram-positive rods.

d. long, thin, gram-positive rods.

ANSWER:

(Level 1, Objective 5)

9. Lactobacillus spp. typically produce colonies that are

a. white and chalky.

b. large, dry, and beta-hemolytic.

c. gray-white, translucent. with subtle beta-hemolysis.

d. pinpoint and alpha-hemolytic.

ANSWER:

(Level 1, Objective 5)

10. Which of the following tests can be used to identify Gardnerella?

1. Catalase negative

2. Oxidase positive

3. Beta hemolysis on human blood agar

4. Beta hemolysis on sheep blood agar

5. Starch fermentation

a. 1, 2, 3, and 5

b. 2, 4, and 5

c. 1 and 3

d. 1, 3, and 5

ANSWER:

(Level 2, Objectives 5 and 6)

11. The only catalase-negative, gram-positive rod capable of producing hydrogen sulfide is also

a. intrinsically vancomycin resistant.

b. esculin positive.

c. motile.

d. gelatinase positive.

ANSWER:

(Level 2, Objectives 5 and 6)

12. A pinpoint, gray-white colony is isolated as >100,000 colonies/mL from the urine of an 89-year-old leukemic patient. The colony stains as palisading gram-positive rods. The organism should be

a. catalase negative and urea positive.

b. catalase positive and urea positive.

c. catalase negative and urea negative.

d. catalase positive and urea negative.

ANSWER:

(Level 2, Objective 6)

13. The gram-positive rod that produces an umbrella motility pattern in semisolid medium is also

a. catalase positive.

b. esculin negative.

c. non-hemolytic.

d. sodium hippurate negative.

ANSWER:

(Level 2, Objective 6)

14. The colonies of Streptococcus agalactiae and Listeria monocytogenes are nearly identical. The two organisms can be differentiated by the

a. sodium hippurate hydrolysis test.

b. CAMP factor test.

c. catalase test.

d. diseases they cause.

ANSWER:

(Level 1, Objective 7)

15. A 20-year-old female presents with pharyngitis. The rapid group A Streptococcus screen is negative, and a blood agar plate is inoculated. After 24 hours incubation, a tiny gray colony with a narrow zone of beta-hemolysis is observed. The microbiologist performs a streptococcal serotype for group A and the result is negative. She should

a. perform a Gram stain and catalase test.

b. finalize the culture as “No Group A Streptococcus isolated.”

c. repeat the streptococcal serotype after boiling the organism suspension.

d. perform an ELEK test.

ANSWER:

(Level 2, Objective 7)

17. Which of the following organisms is not considered pathogenic until it has been infected by a bacteriophage?

a. Bacillus anthracis

b. Corynebacterium pseudotuberculosis

c. C. diphtheriae

d. Actinomyces israelii

ANSWER:

(Level 1, Objective 9)

19. The modified ELEK test is used to

a. detect the metachromatic granules of Corynebacterium diphtheriae.

b. identify C. diphtheriae.

c. detect toxin-producing strains of C. diphtheriae.

d. differentiate C. jeikeium and C. urealyticum.

ANSWER:

(Level 1, Objective 9)

20. A leg wound from a 15-year-old male football player yields:

Heavy growth of Staphylococcus aureus

Light growth of Staphylococcus not S. aureus

Light growth of Diphtheroids

Light growth of viridians Streptococcus

The microbiologist should

a. report the Staphylococcus aureus and call the other organisms normal skin flora.

b. report each of the organisms isolated individually.

c. perform further testing on the diphtheroids.

d. report all four organisms as normal skin flora.

ANSWER:

(Level 2, Objective 10)

21. Individuals infected with toxigenic Corynebacterium diphtheriae must be treated with

a. penicillin.

b. DPT vaccine.

c. antitoxin.

d. a and c.

e. b and c.

ANSWER:

(Level 1, Objective 11)

22 A gray-white colony displaying subtle beta-hemolysis is isolated from a cerebrospinal fluid. The organism is a gram-positive rod that is catalase positive, motile at room temperature, and esculin positive. Which patient history is most likely associated with this culture?

a. 45-year-old female weaver

b. 2-day-old infant male delivered at 32 weeks gestation

c. 33-year-old female with recent dental surgery

d. 58-year-old cattle farmer

ANSWER:

(Level 2, Objective 12)

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in