Question

Session 1: 1 flask with halo broth medium(25% NaCl) and Unprocessed salt

Session 2: 1 halo plate spread with halo broth medium(25% NaCl) and Unprocessed salt

Session 3: 1 halo plate, nutrient agar plate, potato dextrose plate. Antibiotic Discs: anisomycin, cycloheimide, streptomycin and thiostrepton

Results from Session 3: Table below

	Streptomycin	Anisomycin	Cycloheximide	Thiostrepton
Staphylococcus	+	-	-	+
Saccharomyces	-	+	+	-
Halophilic Isolate	-	+	-	+

Answer the following the question on Isolation of Halophilic Archaea.

By what 2 criteria can you be certain that the pigmented organism that you isolated is not an alga?