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You are growing bacteria with an inducible operon for the use of lactose as a source of energy. The culture medium has not lactose but at one-time point you add lactose to the medium. Which temporal sequence of events do you expect to see over time?

a) 1. Increase in Beta-Galactosidase mRNA. 2. Increase in Beta-Galactosidase. 3. Increase in lactose consumption.

b) 1. Increase in Beta-Galactosidase. 2. Decrease in Beta-Galactosidase mRNA. 3. Decrease in lactose consumption.

c) 1. Increase in lactose consumption. 2. Increase in Beta-Galactosidase mRNA. 3. Decrease in Beta-Galactosidase

d) 1. Decrease in Beta-Galactosidase mRNA. 2. Increase in lactose consumption. 3. Decrease in Beta-Galactosidase.

e) 1. Decrease in Beta-Galactosidase mRNA. 2. Decrease in Beta-Galactosidase. 3. Decrease in lactose consumption.

Which cellular proceses occur as a consequence of differential control of gene expression in eukaryotic cell?

a) cell differentiation

b) differential translation

c) differential polymerization

d) DNA replication

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Beverley Smith
Beverley SmithLv2
30 Sep 2019

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Related questions

Cell Biology (#1-5)

1) What does a non-synonymous DNA mutation cause?

a) No amino acid change

b) A change in the reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

2) What does a non-sense DNA mutation cause?

a) No amino acid change b) Alters the normal reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

3) If you sequence the genome of 100 cells from different tissues of the same multicellular organism. How do you expect the sequences to be?

a) 10% identical c)100% identical

b) 50% identical d) 100% different e) will depend on the tissue selected

4) Multicellular organisms are comprised of a variety of cells with different morphologies and different functions. Which is the underlying mechanism that leads to the generation of diverse cell in one organism?

a) The expression of all genes in all cell types

b) The duplication of the genome in certain cell types

c) The elimination of specific group of genes from different cell types

d) The differential expression of particular sets of genes requires for a particular cellular phenotype

e) All of the above

5)You are growing bacteria which can use lactose and glucose. The culture medium has lactose, which induces transcription of the enzymes required to use lactose. If you add glucose to the medium. What do you expect to happen?

a) The bacteria will keep using lactose

b) The bacteria will shut down the Lac operon and use glucose

c) The bacteria will stop growing

d) The bacteria will die

e) The bacteria will use both lactose and glucose

please answer all the questions answering only one question is not helpful!
Question 1

0 / 1 pts

Which of the following E. coli strains with the lac genotypes will synthesize beta-galactosidase in the presence of lactose?

Note: Superscript wouldn't work in the answers, so for example, lacIs means lacIs(superrepressor of lacI)

lacI– lacP– lacO– lacZ– lacY–

lacIs lacP+ lacO+ lacZ+ lacY– / lacI+ lacP+ lacO+ lacZ– lacY+

lacIs lacP– lacO+ lacZ– lacY+ / lacI+ lacP+ lacO+ lacZ+ lacY+

lacI– lacP– lacO+ lacZ+ lacY+

lacI+ lacP+ lacO+ lacZ+ lacY+

Question 2

0 / 1 pts

Some mutations in the trp 5' UTR region increase termination by the attenuator. An example of a mutation that may have this effect is a

Choose the best answer!

mutation that changes the bases in region 3 of the 5' UTR, such that they no longer pair with region 2 or 4.

deletion of the two tryptophan codons in the 5' UTR leader peptide.

deletion in region 2 in the 5' UTR that prevents it from pairing with region 3.

The second and third answers are correct.

The first, second, and third answers are correct.

IncorrectQuestion 3

0 / 1 pts

Several examples of antisense RNA regulating translation in bacterial cells have been discovered. If you wanted to design a construct to artificially control translation of a bacterial gene with antisense RNA, what type of sequences should the antisense RNA contain?

A sequence complementary to the operator of an operon.

A sequence complementary to a 5' UTR.

A sequence complementary to the Shine-Dalgarno site of the mRNA.

The first and second choices are correct.

The second and third choices are correct.

Question 4

0 / 1 pts

Which of the following correctly describes the function of microRNAs?

Choose the best answer.

MicroRNAs are encoded by genes in the genome of an organism.

MicroRNAs form mRNA which encodes a protein repressor for another gene.

MicroRNAs encode a mRNA that base pairs with itself, forming double stranded RNA.

MicroRNAs are cut into small pieces, then bound by the complex of proteins called RISC to repress the translation of other mRNAs in the cell.

The first and second answers are correct.

The first, third, and fourth answers are correct.

The third and fourth answers are correct.

True or False????? HELP

1. In the field of epidemiology, increased prevalence of a disease in a population is the universal signal that disease control and treatment are not effective.

2. Cytoplasmic membranes of prokaryotes typically contain more proteins than cytoplasmic membranes of eukaryotes microorganisms.
3. Sanger (or dideoxy) DNA sequencing produces relatively short DNA sequence data, but is by far the least expensive DNA sequencing method currently available.
4. The (italicizes) abbreviation lac Z refers explicitly to the beta galactosidase protein/ enzyme.
5. Archaeal pathogens have been linked to some of the most virulent infectious diseases. 6. A bacterial mutant that is unable to make an amino acid required for growth (relative to its wild-type strain is termed an auxotroph.
7. When counting plaques to enumerate phage particles (as in the BIOL2026 Laboratory), the plaque represents lacK, not presence, of bacterial growth.
8. Because of competition with plant roots for nutrients, microbial numbers in the rhizosphere are typically much lower than in surrounding bulk soil.
9. Chemolithoautotrophy is the strategy that facilitates the existence of biological communities deep beneath the earth’s surface and near hydrothermal vents.

10. In a primary treatment (1 °) system of a municipal sewage/ wastewater treatment plant, the main goal is to remove ammonium (NH4+) using specific bacterial enrichment cultures.
11. The small intestine in human is the main site of microbial breakdown of partially digested food.
12. Root nodules of legumes contain nitrogen fixing bacteria.
13. Anoxygenic photosynthesis involves using/ removing electrons from H2O.
14. Algae and Cyanobacteria utilize generally the same two-photosystem biochemical mechanisms in the light- reaction of photosynthesis.
15. In bacteria, the only known reason proton motive force is generated is to power ATP synthase in cellular respiration/ oxidative phosphorylation.
16. Anaerobic respiration is only carried out by prokaryotes, however fermentation can be carried out by both prokaryote and eukaryal microorganism.

17. The general structure and biochemical “motors” of flagella are similar between Bacteria and eukaryotic cells, but fundamentally different in the Archaea.
18. Antibiotic resistance genes appeared in microbes (for the first time ever) only after human .. producing and releasing massive amounts of antibiotics to the environment.
19. Experiment by Esther Lederberg, and then later by Luria and Delbruck (that won them a Nobel prize), illustrated that genetic mutations in bacteria arose in the absence of selective pressures, rather than in response to selection.
20. Quaternary sensing is a mechanism used by bacteria to sense other bacteria and regulate gene expression accordingly (e.g. In the case of expressing the liac operon or initiating EPS production in biofilms).

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